| Full text | |
| Author(s): |
Simionato, Ana Valeria C.
[1, 2, 3]
;
Silva-Stenico, Maria Estela
[4]
;
Tsai, Siu Mui
[4]
;
Carrilho, Emanuel
[1, 3]
Total Authors: 4
|
| Affiliation: | [1] Univ Estadual Campinas, Inst Nacl Ciencia & Tecnol Bioanalit, BR-13083970 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Grp Toxicol Alimentos & Farmacos, BR-13083970 Campinas, SP - Brazil
[3] Univ Sao Paulo, Grp Bioanalit Microfabricacao & Separacoes, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil
[4] Univ Sao Paulo, Lab Biol Celular & Mol, Ctr Energia Nucl Agr, BR-13416000 Piracicaba, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Journal of the Brazilian Chemical Society; v. 21, n. 4, p. 635-641, 2010. |
| Web of Science Citations: | 1 |
| Abstract | |
Siderophore molecules from grapevine Xylella fastidiosa were investigated. Such metabolites sequester iron, an essential element, from the host, making them a potential factor of pathogenicty. In an iron-limited medium, siderophores were detected in culture plates of X. fastidiosa containing the complex Chromeazurol S (CAS). A combination of different instrumental analyses was used for siderophore(s) characterization, such as: immobilized metal affinity chromatography (IMAC), micelar electrokinetic capillary chromatography (MEKC) and electrospray-mass spectrometry (ESI-MS). The results show that grapevine X. fastidiosa produced siderophore(s), as confirmed by the CAS plate assay. The extraction of the compound(s) using IMAC with immobilized Fe3+ was important for analyte purification. The chelator was not separated by capillary zone electrophoresis, indicating the possibility of a neutral compound under the investigated pHs. MEKC runs presented a different peak (when compared to the control analysis) which represented a slightly hydrophobic compound. Mass spectrometry showed that the compound(s) may have a relative molecular mass within the expected range for siderophore molecules, such as 875, 1004 and 1092 Da. (AU) | |
| FAPESP's process: | 08/57805-2 - Institute of Bioanalytics |
| Grantee: | Lauro Tatsuo Kubota |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 99/09177-1 - Biodiversity of endophytic microorganisms and their biotechnological potential |
| Grantee: | Itamar Soares de Melo |
| Support Opportunities: | BIOTA-FAPESP Program - Thematic Grants |