High-Density Lipoprotein Inhibits the Uptake of Modified Low-Density Lipoprotein and the Expression of CD36 and Fc gamma RI

Autor(es)

Teixeira Carvalho, Marcia Dias ^{[1, 2]} ; Vieira Vendrame, Celia Maria ^[2] ; Jacon Ketelhuth, Daniel Francisco ^[1] ; Yamashiro-Kanashiro, Edite Hatsumi ^[2] ; Goto, Hiro ^{[2, 3]} ; Gidlund, Magnus ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Ciencias Biomed, Lab Imunofisiopatol, BR-05403000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Lab Soroepidemiol & Imunobiol, Inst Trop Med Sao Paulo, BR-05403000 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Prevent Med, Fac Med, BR-05403000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ATHEROSCLEROSIS AND THROMBOSIS; v. 17, n. 8, p. 844-857, 2010.
• Citações Web of Science: 7

Resumo

Aim: Modified low-density lipoprotein (mLDL), mainly upon oxidative and enzymatic modification, is the major atherogenic lipoprotein. Conversely, high-density lipoprotein (HDL) is considered anti-atherogenic because of its ability to remove cholesterol. The aim of this work was to analyze both the influence of HDL on the uptake of mLDL and the expression of CD36 and Fc gamma I receptors on monocytic cell lines during cell differentiation. Methods: Uptake of fluorescein isothiocyanate (FITC)-conjugated LDL and FITC-conjugated mLDL, i.e., copper-oxidized LDL (oxLDL) or trypsin enzyme modified LDL (enzLDL), was analyzed, as well as the expression of CD36 and Fc gamma RI in THP-1 and U937 cells, using flow cytometry. Results: HDL inhibited the uptake of mLDL, which varied in degree depending on the cell line or type of mLDL. Further, HDL rapidly decreased CD36 and Fc gamma RI involved in the uptake of mLDL. Conclusions: We demonstrate that modified LDL promotes specific LDL receptor-independent uptake by monocytic cell lines, and that the uptake of LDL and enzLDL is less than that of oxLDL. In this process, HDL diminishes the uptake of LDL or mLDL, which may involve the down-regulation of receptors (CD36 and Fc gamma I). This regulatory process represents another way by which HDL can be anti-atherogenic and it depends on the type of modification of LDL and the stage of differentiation of monocytes to macrophages. (AU)

• Processo FAPESP: 01/00969-4 - Separação de alta resolução de lipoproteína de baixa densidade (LDL) após modificações por processos oxidativos (LDLox) e enzimáticos (LDLenz): avaliação das propriedades imunoestimulatórias da LDL modificada nos monócitos/macrófagos e interação com HDL
• Beneficiário: Márcia Dias Teixeira Carvalho
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado