Regulation of Radial Glial Motility by Visual Experience

Autor(es)

Tremblay, Marc ^{[1, 2, 3]} ; Fugere, Vincent ^[4] ; Tsui, Jennifer ^{[1, 2]} ; Schohl, Anne ^[1] ; Tavakoli, Aydin ^{[1, 2, 3]} ; Travencolo, Bruno A. N. ^[5] ; Costa, Luciano da F. ^[5] ; Ruthazer, Edward S. ^{[1, 2, 3, 4]}

Afiliação do(s) autor(es)

^[1] McGill Univ, Montreal Neurol Inst, Dept Neurol & Neurosurg, Montreal, PQ H3A 2B4 - Canada

^[2] McGill Univ, Montreal Neurol Inst, McGill Program NeuroEngn, Montreal, PQ H3A 2B4 - Canada

^[3] McGill Univ, Montreal Neurol Inst, McGill Grad Program Neurosci, Montreal, PQ H3A 2B4 - Canada

^[4] McGill Univ, Montreal Neurol Inst, Dept Psychol, Montreal, PQ H3A 2B4 - Canada

^[5] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NEUROSCIENCE; v. 29, n. 45, p. 14066-14076, NOV 11 2009.
• Citações Web of Science: 21

Resumo

Radial glia in the developing optic tectum express the key guidance molecules responsible for topographic targeting of retinal axons. However, the extent to which the radial glia are themselves influenced by retinal inputs and visual experience remains unknown. Using multiphoton live imaging of radial glia in the optic tectum of intact Xenopus laevis tadpoles in conjunction with manipulations of neural activity and sensory stimuli, radial glia were observed to exhibit spontaneous calcium transients that were modulated by visual stimulation. Structurally, radial glia extended and retracted many filopodial processes within the tectal neuropil over minutes. These processes interacted with retinotectal synapses and their motility was modulated by nitric oxide (NO) signaling downstream of neuronal NMDA receptor (NMDAR) activation and visual stimulation. These findings provide the first in vivo demonstration that radial glia actively respond both structurally and functionally to neural activity, via NMDAR-dependent NO release during the period of retinal axon ingrowth. (AU)

• Processo FAPESP: 05/00587-5 - Modelagem por redes (grafos) e técnicas de reconhecimento de padrões: estrutura, dinâmica e aplicações
• Beneficiário: Roberto Marcondes Cesar Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 07/02938-5 - Métodos computacionais para caracterização e análise de neurônios e glias
• Beneficiário: Bruno Augusto Nassif Travençolo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado