Molecular cloning and characterization of a ripening-induced polygalacturonase related to papaya fruit softening

Autor(es)

Fabi, Joao Paulo ^[1] ; Cordenunsi, Beatriz Rosana ^[1] ; Seymour, Graham B. ^[2] ; Lajolo, Franco M. ^[1] ; Oliveira do Nascimento, Joao Roberto ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, FCF, Dept Alimentos & Nutr Expt, Lab Quim Bioquim & Biol Mol Alimentos, BR-05508900 Sao Paulo - Brazil

^[2] Univ Nottingham, Plant Sci Div, Loughborough LE12 5RD, Leics - England

• Tipo de documento: Artigo Científico
• Fonte: Plant Physiology and Biochemistry; v. 47, n. 11-12, p. 1075-1081, NOV-DEC 2009.
• Citações Web of Science: 28

Resumo

Pulp softening is one of the most remarkable changes during ripening of papaya (Carica papaya) fruit and it is a major cause for post-harvest losses. Although cell wall catabolism has a major influence on papaya fruit, quality information on the gene products involved in this process is limited. A full-length polygalacturonase cDNA (cpPG) was isolated from papaya pulp and used to study gene expression and enzyme activity during normal and ethylene-induced ripening and after exposure of the fruit to 1-MCP. Northern-blot analysis demonstrated that cpPG transcription was strongly induced during ripening and was highly ethylene-dependent. The accumulation of cpPG transcript was paralleled by enzyme activity, and inversely correlated to the pulp firmness. Preliminary in silica analysis of the cpPG genomic sequence revealed the occurrence of putative regulatory motifs in the promoter region that may help to explain the effects of plant hormones and non-abiotic stresses on papaya fruit firmness. This newly isolated cpPG is an important candidate for functional characterization and manipulation to control the process of pulp softening during papaya ripening. (C) 2009 Elsevier Masson SAS. All rights reserved. (AU)

• Processo FAPESP: 02/12452-9 - Metabolismo de carboidratos durante o amadurecimento de frutos: aplicações da genômica funcional
• Beneficiário: Franco Maria Lajolo
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 07/56515-8 - Clonagem, expressão e caracterização molecular das enzimas pectinolíticas envolvidas no amolecimento da polpa durante o amadurecimento do mamão papaia
• Beneficiário: Joao Paulo Fabi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado