Polyploidy in atypical grade II choroid plexus papilloma of the posterior fossa

Autor(es)

Brassesco, Maria Sol ^[1] ; Valera, Elvis Terci ^[1] ; Neder, Luciano ^[2] ; Castro-Gamero, Angel Mauricio ^[3] ; Arruda, Darlene ^[2] ; Machado, Helio Rubens ^[4] ; Sakamoto-Hojo, Elza Tiemi ^{[2, 5]} ; Tone, Luiz Gonzaga ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Pediat, Div Pediat Oncol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Pathol, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Surg & Anat, Div Neurosurg, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Fac Philosophy Sci & Letters, Dept Biol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NEUROPATHOLOGY; v. 29, n. 3, p. 293-298, JUN 2009.
• Citações Web of Science: 20

Resumo

Cytogenetic studies of choroid plexus tumors, particularly for atypical choroid plexus papillomas, have been rarely described. In the present report, the cytogenetic investigation of an atypical choroid plexus papilloma occurring at the posterior fossa of a 16-year-old male is described. Comparative genome hybridization analysis demonstrated gains of genetic material from almost all chromosomes. Chromosome losses involved 19p, regional losses at chromosome X and loss of chromosome Y. The presence of polyploid cells was confirmed by fluorescence in situ hybridization analysis with probes directed to centromeric regions. Furthermore, the microscopic analysis of cultures showed nuclear buds, nucleoplasmic bridges, and micronuclei in 23% of tumor cells suggesting the presence of complex chromosomal abnormalities. Previous cytogenetic studies on choroid plexus papillomas showed either normal, hypodiploid or hyperdiploid karyotypes. To the best of our knowledge, this is the first report of polyploidy in choroid plexus papilloma of intermediate malignancy grade. Although the mechanisms beneath such genome duplication remain to be elucidated, the observed abnormal nuclear shapes indicate constant restructuring of the tumor's genome and deserves further investigation. (AU)

• Processo FAPESP: 07/54236-4 - Analise do perfil genomico em neoplasias da infancia atraves da hibridizacao genomica comparativa.
• Beneficiário: Luiz Gonzaga Tone
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/04827-3 - Análise de aneuploidias por hibridação genômica comparativa (CGH) em pacientes pediátricos portadores de leucemia linfóide aguda
• Beneficiário: María Sol Brassesco Annichini
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado