Gene Expression Profiling of Cultured Cells From Brainstem of Newborn Spontaneously Hypertensive and Wistar Kyoto Rats

Autor(es)

Ferrari, Merari F. R. ^[1] ; Reis, Eduardo M. ^[2] ; Matsumoto, Joao P. P. ^[1] ; Fior-Chadi, Debora R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, BR-05508090 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Cellular and Molecular Neurobiology; v. 29, n. 3, p. 287-308, MAY 2009.
• Citações Web of Science: 6

Resumo

The spontaneously hypertensive rat (SHR) is a good model to study several diseases such as the attention-deficit hyperactivity disorder, cardiopulmonary impairment, nephropathy, as well as hypertension, which is a multifactor disease that possibly involves alterations in gene expression in hypertensive relative to normotensive subjects. In this study, we used high-density oligoarrays to compare gene expression profiles in cultured neurons and glia from brainstem of newborn normotensive Wistar Kyoto (WKY) and SHR rats. We found 376 genes differentially expressed between SHR and WKY brainstem cells that preferentially map to 17 metabolic/signaling pathways. Some of the pathways and regulated genes identified herein are obviously related to cardiovascular regulation; in addition there are several genes differentially expressed in SHR not yet associated to hypertension, which may be attributed to other differences between SHR and WKY strains. This constitute a rich resource for the identification and characterization of novel genes associated to phenotypic differences observed in SHR relative to WKY, including hypertension. In conclusion, this study describes for the first time the gene profiling pattern of brainstem cells from SHR and WKY rats, which opens up new possibilities and strategies of investigation and possible therapeutics to hypertension, as well as for the understanding of the brain contribution to phenotypic differences between SHR and WKY rats. (AU)

• Processo FAPESP: 06/00650-1 - Efeitos da nicotina sobre a regulação gênica de sistemas de neurotransmissão envolvidos no controle cardiovascular e gênese da hipertensão em ratos geneticamente hipertensos
• Beneficiário: Merari de Fátima Ramires Ferrari
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado