Evaluation of the cytotoxicity and genotoxicity of curcumin in PC12 cells

Autor(es)

Mendonca, Leonardo Meneghin ; dos Santos, Graciela Cristina ; Antonucci, Gilmara Ausech ; dos Santos, Antonio Cardozo ; Pires Bianchi, Maria de Lourdes ; Greggi Antunes, Lusania Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Lab Nutrigenom, BR-14040903 Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS; v. 675, n. 1-2, p. 29-34, Apr. 2009.
• Citações Web of Science: 33
• Assunto(s): Quimioterapia Síndromes neurotóxicas Micronúcleo germinativo Cisplatino Curcumina

Resumo

Neurotoxicity induced by reactive oxygen species can appear as an adverse effect of chemotherapy treatment with platinum corn pounds. such as cisplatin. The use of this drug in clinical practice is limited due to its adverse effects, including nephrotoxicity, ototoxicity. neurotoxicity and genotoxicity. Functional foods or nutraceuticals have demonstrated potential neuroprotective activity in several experiments and models. This study aimed to investigate the possible cytotoxicity and genotoxicity/antigenotoxic effects of curcumin in PC12 cells exposed to cisplatin. Cell viability and genotoxicity/antigenotoxicity were evaluated by the MTT assay and micronucleus test, respectively. PC12 cells were treated with different concentrations of cisplatin and curcumin (0.5-128 mu g/mL). Analysis of the results showed that high concentrations of curcumin were cytotoxic and increased micronuclei frequency compared to the control group. In the associated treatments, at all three concentrations evaluated. curcumin significantly reduced the total frequency of micronuclei induced by cisplatin. Determining the cytotoxic and genotoxic/antigenotoxic effects of this frequently used antioxidant in a neuronal model is important to assess possible hazards when combined with other chemical agents, including chemotherapy drugs used in cancer therapy. (C) 2009 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 08/53947-7 - Neurotoxicidade induzida pelo quimioterápico cisplatina: possíveis efeitos citoprotetores dos antioxidantes da dieta curcumina e coenzima Q10
• Beneficiário: Lusânia Maria Greggi Antunes
• Modalidade de apoio: Auxílio à Pesquisa - Regular