Distinct effects of acute and chronic sleep loss on DNA damage in rats

Autor(es)

Andersen, M. L. ^[1] ; Ribeiro, D. A. ^[2] ; Bergamaschi, C. T. ^{[2, 3]} ; Alvarenga, T. A. ^[1] ; Silva, A. ^[1] ; Zager, A. ^[1] ; Campos, R. R. ^[3] ; Tufik, S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, UNIFESP, Dept Psychobiol, BR-11060001 Santos, SP - Brazil

^[2] Univ Fed Sao Paulo, UNIFESP, Dept Biosci, BR-11060001 Santos, SP - Brazil

^[3] Univ Fed Sao Paulo, UNIFESP, Dept Cardiovasc Physiol, BR-11060001 Santos, SP - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY; v. 33, n. 3, p. 562-567, APR 30 2009.
• Citações Web of Science: 36

Resumo

The aim of this investigation was to evaluate genetic damage induced in male rats by experimental sleep loss for short-term (24 and 96 h) and long-term (21 days) intervals, as well as their respective recovery periods in peripheral blood, brain, liver and heart tissue by the single cell gel (comet) assay. Rats were paradoxically deprived of sleep (PSD) by the platform technique for 24 or 96 h, or chronically sleep-restricted (SR) for 21 days. We also sought to verify the time course of their recovery after 24 h of rebound sleep. The results showed DNA damage in blood cells of rats submitted to PSD for 96 h. Brain tissue showed extensive genotoxic damage in PSD rats (both 24 and 96 h), though the effect was more pronounced in the 96 h group. Rats allowed to recover from the PSD-96 h and SR-21 days treatments showed DNA damage as compared to negative controls. Liver and heart did not display any genotoxicity activity. Corticosterone concentrations were increased after PSD (24 and 96 h) relative to control rats, whereas these levels were unaffected in the SR group. Collectively, these findings reveal that sleep loss was able to induce genetic damage in blood and brain cells, especially following acute exposure. Since DNA damage is an important step in events leading to genomic instability, this study represents a relevant contribution to the understanding of the potential health risks associated with sleep deprivation. (C) 2009 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 06/58274-5 - Influencia da restricao alimentar no comportamento sexual de ratos machos.
• Beneficiário: Tathiana Aparecida Fernandes de Alvarenga
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 07/01228-4 - Carcinogênese bucal quimicamente induzida pela 4-nitroquinolina 1-óxido em ratos: possíveis biomarcadores envolvidos em sua patogênese
• Beneficiário: Daniel Araki Ribeiro
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 98/14303-3 - Center for Sleep Studies
• Beneficiário: Sergio Tufik
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs