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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Distinct effects of acute and chronic sleep loss on DNA damage in rats

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Author(s):
Andersen, M. L. [1] ; Ribeiro, D. A. [2] ; Bergamaschi, C. T. [2, 3] ; Alvarenga, T. A. [1] ; Silva, A. [1] ; Zager, A. [1] ; Campos, R. R. [3] ; Tufik, S. [1]
Total Authors: 8
Affiliation:
[1] Univ Fed Sao Paulo, UNIFESP, Dept Psychobiol, BR-11060001 Santos, SP - Brazil
[2] Univ Fed Sao Paulo, UNIFESP, Dept Biosci, BR-11060001 Santos, SP - Brazil
[3] Univ Fed Sao Paulo, UNIFESP, Dept Cardiovasc Physiol, BR-11060001 Santos, SP - Brazil
Total Affiliations: 3
Document type: Review article
Source: PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY; v. 33, n. 3, p. 562-567, APR 30 2009.
Web of Science Citations: 36
Abstract

The aim of this investigation was to evaluate genetic damage induced in male rats by experimental sleep loss for short-term (24 and 96 h) and long-term (21 days) intervals, as well as their respective recovery periods in peripheral blood, brain, liver and heart tissue by the single cell gel (comet) assay. Rats were paradoxically deprived of sleep (PSD) by the platform technique for 24 or 96 h, or chronically sleep-restricted (SR) for 21 days. We also sought to verify the time course of their recovery after 24 h of rebound sleep. The results showed DNA damage in blood cells of rats submitted to PSD for 96 h. Brain tissue showed extensive genotoxic damage in PSD rats (both 24 and 96 h), though the effect was more pronounced in the 96 h group. Rats allowed to recover from the PSD-96 h and SR-21 days treatments showed DNA damage as compared to negative controls. Liver and heart did not display any genotoxicity activity. Corticosterone concentrations were increased after PSD (24 and 96 h) relative to control rats, whereas these levels were unaffected in the SR group. Collectively, these findings reveal that sleep loss was able to induce genetic damage in blood and brain cells, especially following acute exposure. Since DNA damage is an important step in events leading to genomic instability, this study represents a relevant contribution to the understanding of the potential health risks associated with sleep deprivation. (C) 2009 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 07/01228-4 - Medium-term oral carcinogenesis assay induced by 4-nitroquinoline 1-oxide in rats: putative biomarkers involved into its pathogenesis
Grantee:Daniel Araki Ribeiro
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 98/14303-3 - Center for Sleep Studies
Grantee:Sergio Tufik
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC