Study co-transporters NaP-IIb in intestinal absorption of phosphorus in uremic rats

• Grant number: 12/03282-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2012
• End date: May 31, 2013
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Vanda Jorgetti
• Grantee: Renata Amaral Marinho de Souza
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Chronic Kidney Disease (CKD) is a health public problem with alterations in the metabolism of phosphorus (P) and complications that increase morbidity and mortality in these patients. Phosphorus is required in a range of cellular functions, such as DNA, RNA, and phospholipid membrane synthesis, generation of ATP in energy metabolism, cell signaling, enzymatic activity, muscle and membrane function, and participation in bone mineralization. In homeostasis, P intake is absorbed by the small intestine, placed in the bone, and filtrated by the kidney, which can reabsorb and excrete according to specific requirements of the organism. In the face of the decline of renal function, the small intestine can be an important focus in the therapeutical control of hyperphosphatemia, a common finding in CKD patients. The aim of this work is according to investigate the effect of the acute and chronic administration of diet with distinct concentrations of P in the regulation of the co-transporters NaP-IIb and PiT1 in the segments of the small intestines of uremic rats Wistar rats, males, submitted nephrectomy 5/6 and submitted the sham-nephrectomy will be used. The animals will be divided in 2 (two) sets of 3 (three) groups in accordance with the concentration of phosphorus in the diet. One of them submitted a nephrectomy and the other control group. After this period, will be used techniques for analysis of the expression of the co-transporters in the intestinal mucosa in the distinct segments. (AU)