Repeated fed-batch cultivation of Arthrospira (Spirulina) platensis using urea as nitrogen source

Autor(es)

Matsudo, Marcelo C. ^[1] ; Bezerra, Raquel P. ^[1] ; Sato, Sunao ^[1] ; Perego, Patrizia ^[2] ; Converti, Attilio ^[2] ; Carvalho, Joao Carlos M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Pharmaceut Technol & Biochem, BR-05508900 Sao Paulo - Brazil

^[2] Univ Genoa, Dept Chem & Proc Engn GB Bonino, I-16145 Genoa - Italy

• Tipo de documento: Artigo Científico
• Fonte: Biochemical Engineering Journal; v. 43, n. 1, p. 52-57, JAN 15 2009.
• Citações Web of Science: 43

Resumo

Arthospira (Spirulina) platensis (Nordstedt) Gomont was autotrophically cultivated for biomass production in repeated fed-batch process using urea as nitrogen source, with the aim of making large-scale production easier, increasing cell productivity and then reducing the production costs. It was investigated the influence or the ratio of renewed volume to total volume (R), the Urea feeding time (t(f)) and the number of successive repealed fed-batch cycles on the maximum cell concentration (X(m)), cell productivity (P(x)), nitrogen-to-cell conversion yield (Y(x/n)), maximum specific growth rate (mu(m)) and protein content of, dry biomass. The experimental results demonstrated chat R=0.80 and t(f) = 6d were the best cultivation conditions, being able to simultaneously ensure, throughout the three fed-batch cycles, the highest average values of three of the five responses (X(m) = 2101 +/- 113 mg L(-1), P(x) = 219 +/- 13 mg L(-1) d(-1) and Y(x/n) = 10.3 +/- 0.8,g g(-1)). (C) 2008 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 04/04012-4 - Cultivo de spirulina platensis por processo descontinuo alimentado repetitivo utilizando ureia como fonte de nitrogenio.
• Beneficiário: Marcelo Chuei Matsudo
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 04/04004-1 - Avaliação do cultivo de Spirulina platensis utilizando ureia como fonte de nitrogênio por processo descontínuo alimentado repetitivo
• Beneficiário: João Carlos Monteiro de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular