Gene Expression Profiles in Radiation Workers Occupationally Exposed to Ionizing Radiation

Autor(es)

Fachin, Ana Lucia ^[1] ; Mello, Stephano Spano ^[1] ; Sandrin-Garcia, Paula ^[1] ; Junta, Cristina Moraes ^[1] ; Ghilardi-Netto, Thomaz ^[2] ; Donadi, Eduardo Antonio ^[3] ; da Silva Passos, Geraldo Aleixo ^{[1, 4]} ; Sakamoto-Hojo, Elza Tiemi ^{[1, 5]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, HCFMRP, Ctr Ciencias Imagens & Fis Med, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, FMRP, Dept Clin Med, BR-14049900 Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Fac Odontol Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14049 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF RADIATION RESEARCH; v. 50, n. 1, p. 61-71, JAN 2009.
• Citações Web of Science: 43

Resumo

Ionizing radiation OR) imposes risks to human health and the environment. IR at low doses and low (lose rates has the potency to initiate carcinogenesis. Genotoxic environmental agents such as IR trigger a cascade of signal transduction pathways for cellular protection. In this study, using cDNA microarray technique, we monitored the gene expression profiles in lymphocytes derived from radiation-ex posed individuals (radiation workers). Physical dosimetry records on these patients indicated that the absorbed dose ranged from 0.696 to 39.088 mSv. Gene expression analysis revealed statistically significant transcriptional changes in a total of 78 genes (21 up-regulated and 57 clown-regulated) involved in several biological processes such as ubiquitin cycle (UHRF2 and PIAS1), DNA repair (LIG3, XPA, ERCC5, RAD52, DCLRE1C), cell cycle regulation/proliferation (RHOA, CABLES2, TGFB2, IL16), and stress response (GSTP1, PPP2R5A, DUSP22). Some of the genes that showed altered expression profiles in this study call be used as biomarkers for monitoring the chronic low level exposure in humans. Additionally, alterations in gene expression patterns observed in chronically exposed radiation workers reinforces the need for defining the effective radiation dose that causes immediate genetic damage as well as the long-term effects on genomic instability, including cancer. (AU)

• Processo FAPESP: 01/10995-2 - Expressão gênica diferencial induzida pela radiação ionizante analisada por micro arrays em linfócitos humanos
• Beneficiário: Ana Lucia Fachin Saltoratto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 02/07314-6 - Estudo dos mecanismos de resposta ao dano induzido no DNA pela radiação ionizante e cisplatina em linhagens de fibroblastos humanos
• Beneficiário: Stephano Spanó Mello
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 99/12135-9 - Projeto transcriptoma: análise da expressão gênica em larga escala usando DNA-arrays
• Beneficiário: Geraldo Aleixo da Silva Passos Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Temático