Association between estrogen receptor gene polymorphisms and breast density in postmenopausal women

Autor(es)

de Moura Ramos, E. H. ^[1] ; Martinelli, S. ^[1] ; Silva, I. ^[1] ; Nazario, A. ^[1] ; Facina, G. ^[1] ; Costa, A. ^[1] ; Carvalho, C. ^[1] ; Souza, N. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Gynecol, Escola Paulista Med, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CLIMACTERIC; v. 12, n. 6, p. 490-501, 2009.
• Citações Web of Science: 7

Resumo

Objectives The aim of this study was to evaluate the association between clinical characteristics and polymorphisms HaeIII, MspI and XbaI of the estrogen receptor gene a with postmenopausal mammographic density. Methods A prospective study was performed with 120 women who were not users of hormones and had no identified breast lesions. All of them underwent bilateral mammography; the radiological density was determined by three independent observers, with two subjective evaluations based on the ACR-BIRADS(R) classification of mammographic patterns, 2003, and one computerized evaluation using the gray-scale histogram tool of the Adobe Photoshop(R) 7.0 software. Peripheral blood samples were obtained for DNA extraction, performed according to the GFX(R) Kit protocol (Amersham-Pharmacia). Polymerase chain reaction restriction fragment length polymorphism was carried out for an analysis of the polymorphisms present in intron 1 (HaeIII and XbaI) and in exon 1 (MspI) of the estrogen receptor gene. Results There was a high degree of concordance among the observers in the determination of mammary density (Kappa, Pearson and Spearman, p < 0.001). The associations of clinical characteristics with mammary density were: age (p = 0.04), body mass index (p < 0.0001) and age at menarche (p = 0.02). The relationship between the allele distribution of the polymorphisms and density was: XbaI (p = 0.02), HaeIII (p = 0.65) and MspI (p = 0.65). Conclusions Our data suggested that the polymorphism XbaI may be strongly related to mammographic density. (AU)

• Processo FAPESP: 03/04533-1 - Estudo de polimorfismo em genes responsáveis pela biossíntese, ação e metabolização de esteróides sexuais em afecções ginecológicas estrogênio-dependentes e no climatério
• Beneficiário: Ismael Dale Cotrim Guerreiro da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Temático