| Full text | |
| Author(s): |
de Moura Ramos, E. H.
[1]
;
Martinelli, S.
[1]
;
Silva, I.
[1]
;
Nazario, A.
[1]
;
Facina, G.
[1]
;
Costa, A.
[1]
;
Carvalho, C.
[1]
;
Souza, N.
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Fed Sao Paulo, Dept Gynecol, Escola Paulista Med, Sao Paulo - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | CLIMACTERIC; v. 12, n. 6, p. 490-501, 2009. |
| Web of Science Citations: | 7 |
| Abstract | |
Objectives The aim of this study was to evaluate the association between clinical characteristics and polymorphisms HaeIII, MspI and XbaI of the estrogen receptor gene a with postmenopausal mammographic density. Methods A prospective study was performed with 120 women who were not users of hormones and had no identified breast lesions. All of them underwent bilateral mammography; the radiological density was determined by three independent observers, with two subjective evaluations based on the ACR-BIRADS(R) classification of mammographic patterns, 2003, and one computerized evaluation using the gray-scale histogram tool of the Adobe Photoshop(R) 7.0 software. Peripheral blood samples were obtained for DNA extraction, performed according to the GFX(R) Kit protocol (Amersham-Pharmacia). Polymerase chain reaction restriction fragment length polymorphism was carried out for an analysis of the polymorphisms present in intron 1 (HaeIII and XbaI) and in exon 1 (MspI) of the estrogen receptor gene. Results There was a high degree of concordance among the observers in the determination of mammary density (Kappa, Pearson and Spearman, p < 0.001). The associations of clinical characteristics with mammary density were: age (p = 0.04), body mass index (p < 0.0001) and age at menarche (p = 0.02). The relationship between the allele distribution of the polymorphisms and density was: XbaI (p = 0.02), HaeIII (p = 0.65) and MspI (p = 0.65). Conclusions Our data suggested that the polymorphism XbaI may be strongly related to mammographic density. (AU) | |
| FAPESP's process: | 03/04533-1 - Study of polymorphisms in genes responsible for biosynthesis, action and metabolization of sexual steroids in estrogen-dependent and menopausal gynecological conditions |
| Grantee: | Ismael Dale Cotrim Guerreiro da Silva |
| Support Opportunities: | Research Projects - Thematic Grants |