Molecular Characterization of Strains of Respiratory Syncytial Virus Identified in a Hematopoietic Stem Cell Transplant Outpatient Unit Over 2 Years: Community or Nosocomial Infection?

Autor(es)

Machado, Adriana F. ^{[1, 2]} ; Sallum, Maria Anice M. ^[3] ; Boas, Lucy S. Vilas ^{[1, 2, 4]} ; Tateno, Adriona F. ^{[1, 2]} ; Machado, Clarisse M. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Trop Med, Virol Lab LIM52 HCFMUSP, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med Sci, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Publ Hlth, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Med Sci, BMT Program, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION; v. 14, n. 12, p. 1348-1355, DEC 2008.
• Citações Web of Science: 10

Resumo

Respiratory syncytial virus (RSV) is recognized as the leading cause of nosocomial respiratory infection among hematopoietic stem cell transplant (HSCT) recipients, causing considerable morbidity and mortality. RSV is easily transmitted by contact with contaminated surfaces, and in HSCT units, more than 50% of RSV infections have been characterized as of nosocomial origin. From April 2001 to October 2002, RSV was identified by direct immunofluorescent assay in 42 symptomatic HSCT recipients. Seven RSV strains from 2001 and 12 RSV strains from 2002 were sequenced. RNA extraction, cDNA synthesis, and seminested polymerase chain reaction (PCR) with primers complementary to RSV genes G and F were pet-formed. PCR products were analyzed by nucleotide sequencing of the C-terminal region of gene G for typing (in group A or B). Of the 7 strains analyzed in 2001, only 2 belonged to group B; the other 5 belonged to group A. Of these 7 strains, 3 were identical and were from recipients receiving outpatient care. In 2002, of the 12 strains analyzed, 3 belonged to group A and the other 9 belonged to group B. Of these 9 strains, 7 were genetically identical and were also from recipients receiving outpatient care. Therefore, multiple strains of RSV cocirculated in the hematopoietic stem cell transplant units (ward and outpatient units) between 2001 and 2002. Nosocomial transmission was more likely to occur at the HSCT outpatient unit than in the HSCT ward. Infection control practices should also be implemented in the outpatient setting. (AU)

• Processo FAPESP: 01/11087-2 - Infecção pelo vírus sincicial respiratório em receptores de transplante de medula óssea e em crianças infectadas pelo vírus da imunodeficiência humana
• Beneficiário: Clarisse Martins Machado
• Modalidade de apoio: Auxílio à Pesquisa - Regular