Ribotyping, biotyping and capsular typing of Haemophilus influenzae strains isolated from patients in Campinas, southeast Brazil

Autor(es)

Lancellotti, Marcelo ^[1] ; de Pace, Fernanda ^[1] ; Stehling, Eliana Guedes ^[1] ; Barisson Villares, Maria Cecilia ^[2] ; Brocchi, Marcelo ^[1] ; da Silveira, Wanderley Dias ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Microbiol & Immunol, Inst Biol, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Dept Clin Pathol, Med Sch Hosp, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Infectious Diseases; v. 12, n. 5, p. 430-437, OCT 2008.
• Citações Web of Science: 2

Resumo

Forty-five Haemophilus influenzae strains isolated from patients were characterized based on biochemical characteristics. Their capsular types were determined by polymerase chain reaction (PCR); they were compared, using two molecular methods [ribotyping with a specific DNA probe amplified from the 16S rDNA region from H. influenzae and through restriction fragment length polymorphism (RLFP) of an amplified 16S DNA region]. The strains were better discriminated by the ribotyping technique that used the 16S probe and by the combination of both techniques. Biotypes I and IV were the most common, followed by biotypes VI, VIII and III. Biotypes II and VII were not found. Most of the capsular samples were nontypable (89%), with capsular types a and b found in 2 and 9% of the samples, respectively. We concluded that there is a very close genetic identity among pathogenic and non-pathogenic strains. (AU)

• Processo FAPESP: 98/04612-9 - Determinação da estrutura clonal e fatores de patogenicidade de linhagens de Haemophilus influenzae isoladas de pacientes do Hospital das Clínicas da Faculdade de Ciências Médicas da Unicamp
• Beneficiário: Guilherme Linhares Bub
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 00/01864-9 - Determinação das características biológicas e variação clonal de Haemophilus influenzae isolados de pacientes do Hospital de Clínicas da UNICAMP
• Beneficiário: Wanderley Dias da Silveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 00/01882-7 - Determinação das características biológicas e variação clonal de Haemophilus influenzae isolados de pacientes do Hospital das Clínicas da Universidade Estadual de Campinas - Unicamp
• Beneficiário: Marcelo Lancellotti
• Modalidade de apoio: Bolsas no Brasil - Mestrado