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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Ribotyping, biotyping and capsular typing of Haemophilus influenzae strains isolated from patients in Campinas, southeast Brazil

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Author(s):
Lancellotti, Marcelo [1] ; de Pace, Fernanda [1] ; Stehling, Eliana Guedes [1] ; Barisson Villares, Maria Cecilia [2] ; Brocchi, Marcelo [1] ; da Silveira, Wanderley Dias [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Campinas, Dept Microbiol & Immunol, Inst Biol, Campinas, SP - Brazil
[2] Univ Estadual Campinas, Dept Clin Pathol, Med Sch Hosp, Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Brazilian Journal of Infectious Diseases; v. 12, n. 5, p. 430-437, OCT 2008.
Web of Science Citations: 2
Abstract

Forty-five Haemophilus influenzae strains isolated from patients were characterized based on biochemical characteristics. Their capsular types were determined by polymerase chain reaction (PCR); they were compared, using two molecular methods [ribotyping with a specific DNA probe amplified from the 16S rDNA region from H. influenzae and through restriction fragment length polymorphism (RLFP) of an amplified 16S DNA region]. The strains were better discriminated by the ribotyping technique that used the 16S probe and by the combination of both techniques. Biotypes I and IV were the most common, followed by biotypes VI, VIII and III. Biotypes II and VII were not found. Most of the capsular samples were nontypable (89%), with capsular types a and b found in 2 and 9% of the samples, respectively. We concluded that there is a very close genetic identity among pathogenic and non-pathogenic strains. (AU)