Occurrence of Methanogenic Archaea in Highly Polluted Sediments of Tropical Santos-So Vicente Estuary (So Paulo, Brazil)

Autor(es)

Saia, Flavia ^[1] ; Domingues, Mercia ; Pellizari, Vivian ^[1] ; Vazoller, Rosana ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Oceanog, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Current Microbiology; v. 60, n. 1, p. 66-70, JAN 2010.
• Citações Web of Science: 5

Resumo

Little is known about the ability of methanogens to grow and produce methane in estuarine environments. In this study, traditional methods for cultivating strictly anaerobic microorganisms were combined with Fluorescence in situ hybridization (FISH) technique to enrich and identify methanogenic Archaea cultures occurring in highly polluted sediments of tropical Santos-So Vicente Estuary (So Paulo, Brazil). Sediment samples were enriched at 30A degrees C under strict anaerobic and halophilic conditions, using a basal medium containing 2% of sodium chloride and amended with glucose, methanol, and sodium salts of acetate, formate and lactate. High methanogenic activity was detected, as evidenced by the biogas containing 11.5 mmol of methane at 20 days of incubation time and methane yield of 0.138-mmol CH(4)/g organic matter/g volatile suspense solids. Cells of methanogenic Archaea were selected by serial dilution in medium amended separately with sodium acetate, sodium formate, or methanol. FISH analysis revealed the presence of Methanobacteriaceae and Methanosarcina sp. cells. (AU)

• Processo FAPESP: 00/08323-3 - Contribuição à exploração tecnológica dos estudos microbianos realizados no Programa BIOTA-FAPESP: avaliação do potencial da degradação anaeróbia de pentaclorofenol (PCP) em reator anaeróbio horizontal de leito fixo (RAHLF) inoculado com sedimentos do Estuário de Baixada Santista (estado de São Paulo)
• Beneficiário: Flávia Talarico Saia
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 98/05068-0 - Ecologia molecular e taxonomia polifásica de bactérias de importância ambiental e agroindustrial
• Beneficiário: Gilson Paulo Manfio
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático