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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Occurrence of Methanogenic Archaea in Highly Polluted Sediments of Tropical Santos-So Vicente Estuary (So Paulo, Brazil)

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Author(s):
Saia, Flavia [1] ; Domingues, Mercia ; Pellizari, Vivian [1] ; Vazoller, Rosana [2]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Inst Oceanog, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Current Microbiology; v. 60, n. 1, p. 66-70, JAN 2010.
Web of Science Citations: 5
Abstract

Little is known about the ability of methanogens to grow and produce methane in estuarine environments. In this study, traditional methods for cultivating strictly anaerobic microorganisms were combined with Fluorescence in situ hybridization (FISH) technique to enrich and identify methanogenic Archaea cultures occurring in highly polluted sediments of tropical Santos-So Vicente Estuary (So Paulo, Brazil). Sediment samples were enriched at 30A degrees C under strict anaerobic and halophilic conditions, using a basal medium containing 2% of sodium chloride and amended with glucose, methanol, and sodium salts of acetate, formate and lactate. High methanogenic activity was detected, as evidenced by the biogas containing 11.5 mmol of methane at 20 days of incubation time and methane yield of 0.138-mmol CH(4)/g organic matter/g volatile suspense solids. Cells of methanogenic Archaea were selected by serial dilution in medium amended separately with sodium acetate, sodium formate, or methanol. FISH analysis revealed the presence of Methanobacteriaceae and Methanosarcina sp. cells. (AU)

FAPESP's process: 00/08323-3 - Contribution to the technological exploitation of the microbial studies carried out at the BIOTA-FAPESP Program: potential evaluation of the anaerobic degradation of pentachlorophenol (PCP) in an horizontal-flow anaerobic immobilized biomass reactor innoculated with sediments from the Santos-São Vicente Estuary (State of Sâo Paulo)
Grantee:Flávia Talarico Saia
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 98/05068-0 - Molecular ecology and polyphasic taxonomy of bacteria of environmental and agroindustrial importance
Grantee:Gilson Paulo Manfio
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants