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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Quantitative trait loci analysis of citrus leprosis resistance in an interspecific backcross family of (Citrus reticulata Blanco x C. sinensis L. Osbeck) x C. sinensis L. Osb

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Autor(es):
Bastianel, Marines [1] ; Cristofani-Yaly, Mariangela [1] ; de Oliveira, Antonio Carlos [2] ; Freitas-Astua, Juliana [1, 3] ; Franco Garcia, Antonio Augusto [4] ; Vilela de Resende, Marcos Deon [5] ; Rodrigues, Vandeclei [1] ; Machado, Marcos Antonio [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Ctr APTA Citros Sylvio Moreira IAC, BR-13490970 Cordeiropolis, SP - Brazil
[2] Univ Estadual Bahia, Depto Ciencias Nat, Vitoria Da Conquista, BA - Brazil
[3] Embrapa Cassava & Trop Fruits, Cruz Das Almas, BA - Brazil
[4] USP, Escola Super Agron Luiz Queiroz, Dept Genet, Piracicaba, SP - Brazil
[5] Embrapa Florestas, Colombo, PR - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: EUPHYTICA; v. 169, n. 1, p. 101-111, SEP 2009.
Citações Web of Science: 8
Resumo

Leprosis, caused by citrus leprosis virus (CiLV) and transmitted by the tenuipalpid mite Brevipalpus phoenicis, is one of the most important viruses of citrus in the Americas. Sweet oranges (Citrus sinensis L. Osb.) are highly susceptible to CiLV, while mandarins (C. reticulata Blanco) and some of their hybrids have higher tolerance or resistance to this disease. The mechanisms involved in the resistance and its inheritance are still largely unknown. To study the quantitative trait loci (QTL; quantitative trait loci) associated with the resistance to CiLV, progeny analyses were established with 143 hybrid individuals of `Pra' sweet orange (C. sinensis L. Osb.) and `Murcott' tangor (C. reticulata Blanco x C. sinensis L. Osb.) from controlled crossings. Disease assessment of the hybrid individuals was conducted by infesting the plants with viruliferous mites in the field. The experiment consisted of a randomized completely block design with ten replicates. The evaluated phenotypic traits were incidence and severity of the disease on leaves and branches, for a period of 3 years. The MapQTL (TM) v.4.0 software was used for the identification and location of possible QTL associated with resistance to CiLV on a genetic map obtained from 260 AFLP and 5 RAPD markers. Only consistent QTLs from different phenotypic traits and years of evaluation, with the critical LOD scores to determine the presence or absence of each QTL calculated through the random permutation test, were considered. A QTL was observed and had a significant effect on the phenotypic variation, ranging from 79.4 to 84% depending on which trait (incidence or severity) was assessed. This suggests that few genes are involved in the genetic resistance of citrus to CiLV. (AU)

Processo FAPESP: 04/10511-3 - Leprose dos citros: abordagem molecular e funcional da planta, vírus, vetor e suas interações
Beneficiário:Juliana de Freitas Astúa
Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores