Electroacupuncture Inhibits Ethanol-Induced Locomotor Sensitization and Alters homer1A mRNA Expression in Mice

Autor(es)

dos Santos, Jr., Jair Guilherme ^{[1, 2]} ; Filev, Renato ^[3] ; Coelho, Carolina Tesone ^[1] ; Yamamura, Ysao ^[3] ; Mello, Luiz E. ^[1] ; Tabosa, Angela ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Physiol, BR-04101000 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Physiol Sci, FCMSCSP, BR-04101000 Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Orthoped & Traumatol, Div Chinese Med & Acupuncture, BR-04101000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH; v. 33, n. 8, p. 1469-1475, AUG 2009.
• Citações Web of Science: 7

Resumo

Background: Here we investigated the effects of electroacupuncture over locomotor sensitization induced by ethanol in mice. Methods: Adult male Swiss mice were daily injected with ethanol (2 g/kg, i.p.) or saline for 21 days (acquisition phase). After 4 days of withdrawal, all animals were challenged with ethanol (1.4 g/kg, i.p.). The locomotor activity during 30 minutes was accessed just after the ethanol challenge. Electroacupuncture at acquisition, expression, or maintenance phases of locomotor sensitization was provided over ST-36 (Zusanli) or PC-6 (Neiguan) as well as concomitantly over these 2 acupoints. One hour after the challenge with ethanol, the animals were decapitated, the hippocampus, striatum, and prefrontal cortex were dissected, and the expression of homer1A mRNA assessed by PCR. Results: Electroacupuncture provided simultaneously over ST-36 and PC-6 (but not to ST-36 or PC-6 alone) inhibited the acquisition, expression, and maintenance of ethanol-induced locomotor sensitization. In addition, electroacupuncture blocked the diminution of homer1A mRNA expression triggered by ethanol in the acquisition (striatum and prefrontal cortex), expression (hippocampus), and in the maintenance (hippocampus and prefrontal cortex) phases. Conclusion: Electroacupuncture provided concomitantly over ST-36 and PC-6 prevents the sensitization of the mesocorticolimbic pathway induced by ethanol in mice. In addition, these effects were accompanied by changes in the expression of homer1A. We suggest that electroacupuncture effects over ethanol-induced locomotor sensitization are associated to its ability to modulate homer1A expression and glutamatergic plasticity. (AU)

• Processo FAPESP: 05/60052-8 - Estudo sobre o efeito da eletroacupuntura em camundongos submetidos a modelos animais de dependência ao álcool
• Beneficiário: Renato Filev
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 05/55008-0 - Receptores glutamatérgicos ionotrópicos e plasticidade neuronal em modelos de dependência ao etanol em camundongos
• Beneficiário: Jair Guilherme dos Santos Junior
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 05/55626-5 - Estudo sobre o efeito de eletroacupuntura em camundongos submetidos a modelos animais de dependencia ao alcool.
• Beneficiário: Angela Maria Florencio Tabosa
• Modalidade de apoio: Auxílio à Pesquisa - Regular