Differential expression of cytokines, chemokines and chemokine receptors in patients with coronary artery disease

Autor(es)

Dias de Oliveira, Romulo Tadeu ^[1] ; Mamoni, Ronei Luciano ^[1] ; Matos Souza, Jose Roberto ^[2] ; Fernandes, Juliano Lara ^[2] ; Rios, Francisco Jose O. ^[3] ; Gidlund, Magnus ^[3] ; Coelho, Otavio Rizzi ^[2] ; Souza Lima Blotta, Maria Heloisa ^[1]

Afiliação do(s) autor(es)

^[1] State Univ Campinas UNICAMP, Fac Med Sci, Dept Clin Pathol, BR-13083970 Campinas, SP - Brazil

^[2] State Univ Campinas UNICAMP, Fac Med Sci, Dept Internal Med, BR-13083970 Campinas, SP - Brazil

^[3] Univ Sao Paulo USP, Inst Biomed Sci, Dept Immunol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF CARDIOLOGY; v. 136, n. 1, p. 17-26, JUL 24 2009.
• Citações Web of Science: 15

Resumo

Monocytes/macrophages and lymphocytes have a key role in the pathogenesis of atherosclerosis through the production of inflammatory and anti-inflammatory cytokines. We evaluated mRNA expression and protein production of CCL2, CXCL8, CXCL9, CXCL10, IFN-gamma and IL-10 in vitro as well as the expression of the CCR2 and CXCR3 receptors in peripheral blood mononuclear cells (PBMCs) of patients with coronary artery disease (CAD) and healthy controls in the presence or absence of oxidized LDL (oxLDL). Patients with CAD showed higher constitutive expression of CCL2, CXCL8, CXCL9, CXCL10 and IFN-gamma mRNA and, after stimulation with oxLDL, higher expression of CCL2 and CXCL8 mRNA than the control group. We also detected higher levels of CCL2 and CXCL8 in supernatants of oxLDL-stimulated PBMCs from CAD patients than in corresponding supernatants from controls. Patients with CAD had a higher percentage of constitutive CCR2(+) and CXCR3(+) cells after stimulation with oxLDL. Among CAD patients, the main differences between the stable (SA) and unstable angina (UA) groups were lower IL-10 mRNA production in the latter group. Altogether, our data suggest that PBMCs from CAD patients are able to produce higher concentrations of chemokines and cytokines involved in the regulation of monocyte and lymphocyte migration and retention in atherosclerotic lesions. (C) 2008 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 04/01697-6 - Expressao de quimiocinas por celulas mononucleares do sangue periferico de pacientes com angina instavel e estavel.
• Beneficiário: Maria Heloisa Souza Lima Blotta
• Modalidade de apoio: Auxílio à Pesquisa - Regular