| Texto completo | |
| Autor(es): |
Oliveira‚ V. X.
;
Fázio‚ M. A.
;
Miranda‚ M. T. M.
;
da Silva‚ J. M.
;
Bittencourt‚ J. C.
;
Elias‚ C. F.
;
Miranda‚ A.
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Regulatory Peptides; v. 127, n. 1-3, p. 123-132, Apr. 2005. |
| Área do conhecimento: | Ciências Biológicas - Bioquímica |
| Assunto(s): | Obesidade Peptídeos |
| Resumo | |
Leptin presents an important role in energy balance and neuroendocrine control in mammals. In an attempt to identify regions of the leptin molecule responsible for its bioactivity, we have synthesized six peptides based on the protein three-dimensional structure. Fragments were synthesized by the solid-phase methodology, purified by reverse-phase high-performance liquid chromatography (RP-HPLC), and characterized by liquid chromatography–electrospray ionization mass spectrometry (LC/ESI-MS). They were injected intravenously and their ability to induce Fos immunoreactivity (Fos-ir) in rat hypothalamus was compared with that of the recombinant human leptin and saline. Fragment Ac-[Ser117]Lep116–140–NH2 (V) induced Fos-ir in hypothalamic nuclei that express leptin receptor long form. No similar ability was observed for the other five fragments. To investigate whether Fos-ir was induced in the same neuronal group activated by leptin, we proceeded with a dual-label immunohistochemistry for cocaine- and amphetamine-regulated transcript (CART), a neuropeptide related to leptin action in rat hypothalamus. We found that Ac-[Ser117]Lep116–140–NH2 (V) differentially activates CART neurons through the rostrocaudal extension of the arcuate nucleus. These results suggest that this fragment acts in the same group of neurons that mediate leptin response. This approach may offer the basis for the development of leptin-related compounds, having potential application in human or veterinary medicine. (AU) | |
| Processo FAPESP: | 00/03642-3 - Estudo da relação estrutura-atividade de peptídeos biologicamente ativos: leptina, fator de liberação da corticotrofina e gomesina |
| Beneficiário: | Antonio de Miranda |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 99/10413-1 - Peptídeos sintéticos derivados da leptina humana |
| Beneficiário: | Vani Xavier de Oliveira Junior |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 99/03490-0 - Estudo das conexões do núcleo pré-mamilar ventral responsivas a leptin circulante: relação entre o estado nutricional e o comportamento reprodutivo |
| Beneficiário: | Carol Fuzeti Elias |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 03/06022-4 - Papel do neuropeptideo cart hipotalamico como mediador da acao da leptina sobre o controle reprodutivo. |
| Beneficiário: | Carol Fuzeti Elias |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 00/05530-8 - Módulos multiusuários para suporte de técnicas de biologia celular e molecular do Departamento de Anatomia ICB/USP |
| Beneficiário: | Jackson Cioni Bittencourt |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |