| Texto completo | |
| Autor(es): |
Zampronio‚ AR
;
Melo‚ MCC
;
Hopkins‚ SJ
;
Souza‚ GEP
Número total de Autores: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Inflammation Research; v. 49, n. 9, p. 473-479, 2000. |
| Resumo | |
Objective: This study investigated the role of corticotrophin-releasing hormone (CRH) in mediating the fever induced by a novel pre-formed pyrogenic factor (PFPF), using a CRF antagonist in vivo and evaluating the capacity of PFPF to stimulate CRH release from the hypothalamus in vitro. Materials and Methods: Male Wister rats were used. The PFPF, induced following brief incubation of rat peritoneal macrophages with LPS and retained on 10 or 20 kDa MW cut-off membranes, was injected intracerebroventricularly. Fever was monitored using a rectal probe. Hypothalamus tissue was incubated with PFPF to establish its ability to induce CRH release. The CRH was measured by ELISA. Results: PFPF induced a dose-dependent fever that was abolished by boiling or pronase treatment. Whereas both dexamethasone and indomethacin were effective in reducing interleukin- (IL) 1 beta-induced fever, only dexamethasone abolished the fever induced by PFPF The CRH antagonist, alpha-helical CRH9-41, abolished the fever induced by synthetic CRH, IL-8 and PFPF but not tumour necrosis factor-alpha (TNF-alpha). Like IL-1, PFPF was able to induce the release of CRH from rat hypothalamic tissue in vitro. Conclusions: These results suggest that the fever induced by PFPF depends on CRH release but not prostaglandin synthesis. (AU) | |
| Processo FAPESP: | 97/09837-6 - Mecanismos e mediadores envolvidos na integração das respostas inflamatória e febril |
| Beneficiário: | Glória Emília Petto de Souza |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 96/05993-0 - Mecanismos envolvidos na atividade antipirética da dipirona |
| Beneficiário: | Glória Emília Petto de Souza |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Cooperação CNPq-FAPESP |