Transdentinal protective role of sodium ascorbate against the cytopathic effects of H2O2 released from bleaching agents

Autor(es)

Lima, Adriano Fonseca ^[1] ; Rosetti Lessa, Fernanda Campos ; Gasparoto Mancini, Maria Nadir ^[2] ; Hebling, Josimeri ^[3] ; de Souza Costa, Carlos Alberto ^[4] ; Marchi, Giselle Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Sch Dent, Dept Restorat Dent, Piracicaba, SP - Brazil

^[2] Univ Sao Paulo State, UNESP, Sao Jose dos Campos Sch Dent, Dept Physiol Sci, Sao Jose Dos Campos, SP - Brazil

^[3] Univ Sao Paulo State, UNESP, Dept Orthodont & Pediat Dent, Araraquara Sch Dent, BR-14801903 Araraquara, SP - Brazil

^[4] Univ Sao Paulo State, UNESP, Fac Odontol Araraquara, Dept Fisiol & Patol, Araraquara Sch Dent, BR-14801903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology; v. 109, n. 4, p. e70-e76, 2010.
• Citações Web of Science: 21

Resumo

Objectives. The objectives of this study were to evaluate the transdentinal cytotoxicity of 10% and 16% carbamide peroxide gel (CP), as well as the ability of the antioxidant, 10% sodium ascorbate (SA), to protect the odontoblasts in culture. Study design. Human dentin discs of 0.5-mm thickness were obtained and were placed into artificial pulp chambers. MDPC-23 odontoblastlike cells were seeded on pulp surface of the discs and the following groups were established: G1-No Treatment (control), G2-10% SA/6hs, G3-10%/CP6hs, G4-10%SA/6hs+10%CP/6hs, G5-16%CP/6hs, and G6-10%SA/6hs+16%CP/6hs. The cell viability was measured by the MTT assay. Results. In groups where 16% CP was used, decreased cell viability was observed. Conversely, the application of 10% SA on the dentin discs, before the use of the CP, reduced the cytotoxic effects of these products on cells. Conclusions. The 16% CP cause a significant decrease in MDPC-23 cell viability and 10% SA was able to partially prevent the toxic effects of CP. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010; 109: e70-e76) (AU)

• Processo FAPESP: 08/05890-6 - Efeito citotóxico de agente clareador sobre células odontoblastóides
• Beneficiário: Carlos Alberto de Souza Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/58780-8 - Avaliacao da citotoxicidade do peroxido de carbamida e do efeito protetor do ascorbato de sodio sobre celulas odontoblasticas mdpc-23.
• Beneficiário: Adriano Fonseca de Lima
• Modalidade de apoio: Bolsas no Brasil - Mestrado