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(Reference retrieved automatically from Google Scholar through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Transdentinal protective role of sodium ascorbate against the cytopathic effects of H2O2 released from bleaching agents

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Author(s):
Lima, Adriano Fonseca [1] ; Rosetti Lessa, Fernanda Campos ; Gasparoto Mancini, Maria Nadir [2] ; Hebling, Josimeri [3] ; de Souza Costa, Carlos Alberto [4] ; Marchi, Giselle Maria [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Campinas, Piracicaba Sch Dent, Dept Restorat Dent, Piracicaba, SP - Brazil
[2] Univ Sao Paulo State, UNESP, Sao Jose dos Campos Sch Dent, Dept Physiol Sci, Sao Jose Dos Campos, SP - Brazil
[3] Univ Sao Paulo State, UNESP, Dept Orthodont & Pediat Dent, Araraquara Sch Dent, BR-14801903 Araraquara, SP - Brazil
[4] Univ Sao Paulo State, UNESP, Fac Odontol Araraquara, Dept Fisiol & Patol, Araraquara Sch Dent, BR-14801903 Araraquara, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology; v. 109, n. 4, p. e70-e76, 2010.
Web of Science Citations: 21
Abstract

Objectives. The objectives of this study were to evaluate the transdentinal cytotoxicity of 10% and 16% carbamide peroxide gel (CP), as well as the ability of the antioxidant, 10% sodium ascorbate (SA), to protect the odontoblasts in culture. Study design. Human dentin discs of 0.5-mm thickness were obtained and were placed into artificial pulp chambers. MDPC-23 odontoblastlike cells were seeded on pulp surface of the discs and the following groups were established: G1-No Treatment (control), G2-10% SA/6hs, G3-10%/CP6hs, G4-10%SA/6hs+10%CP/6hs, G5-16%CP/6hs, and G6-10%SA/6hs+16%CP/6hs. The cell viability was measured by the MTT assay. Results. In groups where 16% CP was used, decreased cell viability was observed. Conversely, the application of 10% SA on the dentin discs, before the use of the CP, reduced the cytotoxic effects of these products on cells. Conclusions. The 16% CP cause a significant decrease in MDPC-23 cell viability and 10% SA was able to partially prevent the toxic effects of CP. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010; 109: e70-e76) (AU)

FAPESP's process: 08/05890-6 - Cytotoxic effect of a bleaching agent to the odontoblast cell line
Grantee:Carlos Alberto de Souza Costa
Support Opportunities: Regular Research Grants