Inhibition of Streptococcus mutans biofilm accumulation and development of dental caries in vivo by 7-epiclusianone and fluoride

Autor(es)

Murata, Ramiro M. ^[1] ; Branco-de-Almeida, Luciana S. ^[1] ; Franco, Eliane M. ^[1] ; Yatsuda, Regiane ^[1] ; dos Santos, Marcelo H. ^[2] ; de Alencar, Severino M. ^[3] ; Koo, Hyun ^{[4, 5, 6]} ; Rosalen, Pedro L. ^{[1, 4]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Physiol Sci, Fac Dent Piracicaba, Campinas, SP - Brazil

^[2] Univ Fed Alfenas, UNIFAL MG, Dept Pharm, Alfenas, MG - Brazil

^[3] State Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Agrifood Ind Food & Nutr, Sao Paulo, SP - Brazil

^[4] Nat Prod Res Grp Oral Biol NatPROB, Sao Paulo - Brazil

^[5] Univ Rochester, Med Ctr, Ctr Oral Biol, Eastman Dept Dent, Rochester, NY 14627 - USA

^[6] Univ Rochester, Med Ctr, Dept Microbiol & Immunol, Rochester, NY 14627 - USA

• Tipo de documento: Artigo Científico
• Fonte: BIOFOULING; v. 26, n. 7, p. 865-872, 2010.
• Citações Web of Science: 30

Resumo

7-Epiclusianone (7-epi), a novel naturally occurring compound isolated from Rheedia brasiliensis, effectively inhibits the synthesis of exopolymers and biofilm formation by Streptococcus mutans. In the present study, the ability of 7-epi, alone or in combination with fluoride (F), to disrupt biofilm development and pathogenicity of S. mutans in vivo was examined using a rodent model of dental caries. Treatment (twice-daily, 60s exposure) with 7-epi, alone or in combination with 125 ppm F, resulted in biofilms with less biomass and fewer insoluble glucans than did those treated with vehicle-control, and they also displayed significant cariostatic effects in vivo (p < 0.05). The combination 7-epi + 125 ppm F was as effective as 250 ppm F (positive-control) in reducing the development of both smooth-and sulcal-caries. No histopathological alterations were observed in the animals after the experimental period. The data show that 7-epiclusianone is a novel and effective antibiofilm/anticaries agent, which may enhance the cariostatic properties of fluoride. (AU)

• Processo FAPESP: 06/56379-4 - Potencial antimicrobiano de um novo composto poliprenilado extraido da rheedia brasiliensis e sua influencia na expressao proteica de biofilmes de streptococcus mutans.
• Beneficiário: Maria Cristina Volpato
• Modalidade de apoio: Auxílio à Pesquisa - Regular