Growth of recombinant Drosophila melanogaster Schneider 2 cells producing rabies virus glycoprotein in bioreactor employing serum-free medium

Autor(es)

Galesi‚ A.L.L. ; Aguiar‚ M.A. ; Astray‚ R.M. ; Augusto‚ E.F.P. ; Moraes‚ Â.M.

• Tipo de documento: Artigo Científico
• Fonte: Cytotechnology; v. 57, n. 1, p. 73-81, 2008.

Resumo

Drosophila melanogaster Schneider 2 (S2) cells have been increasingly used as a suitable expression system for the production of different recombinant proteins, and the employment of bioreactors for large-scale culture is an important tool for this purpose. In this work, Drosophila S2 cells producing the rabies virus glycoprotein RVGP were cultivated in bioreactor, employing a serum-free medium, aiming an improvement in cell growth and in glycoprotein production. To overcome cell growth limitation commonly observed in stirred flasks, different experiments in bioreactor were performed, in which some system modifications were carried out to attain the desired goal. The study showed that this cell line is considerably sensitive to hydrodynamic forces, and a high cell density (about 16.0 x 10(6) cells mL(-1)) was only obtained when Pluronic F68 (R) percentage was increased to 0.6% (w/v). Despite ammonium concentration affected RVGP production, and also cell growth, an elevated amount of the target protein was obtained, attaining 563 ng 10(-7) cells. (AU)

• Processo FAPESP: 03/00675-6 - Cultivo de células de Drosophila melanogaster em diferentes meios de cultura para a produção de compostos recombinantes de interesse comercial
• Beneficiário: Adriana Lages Lima Galesi
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 02/09482-3 - Expressão de genes heterólogos em células de dípteros: biologia molecular e engenharia de processos
• Beneficiário: Carlos Augusto Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Temático