Inflammatory mediators involved in the nociceptive and oedematogenic responses induced by Tityus serrulatus scorpion venom injected into rat paws.

Autor(es)

Pessini, Andrea C. ^[1] ; Kanashiro, Alexandre ^[1] ; Malvar, David do C. ^[1] ; Machado, Renes R. ^[1] ; Soares, Denis M. ^[1] ; Figueiredo, Maria J. ^[1] ; Kalapothakis, Evanguedes ^[2] ; Souza, Gloria E. P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Farmacol Lab, Dept Quim & Fis, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Biol Geral, BR-31270901 Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Toxicon; v. 52, n. 7, p. 729-736, 2008.
• Citações Web of Science: 19

Resumo

The present study investigated the role of kinins, prostaglandins (PGs) and nitric oxide (NO) in mechanical hypernociception, Spontaneous nociception and paw oedema after intraplantar (ipl) injection of Tityus serrulatus venom (Tsv) in male Wistar rats. Tsv was ipl-injected in doses of 0.01-10 mu g/paw. Pre-treatment (30 min prior) with DALBK (100 nmol/paw) and icatibant (10 nmol/paw), B1 and B2 selective kinin receptor antagonists, L-NAME (50 mg/kg, i.p., a non-selective nitric oxide synthase inhibitor) or celecoxib, selective COX-2 inhibitor, was given 1 h prior per os (5 mg/kg, p.o.), significantly reduced the hypernociceptive response (Von Frey method), the spontaneous nociception (determined by counting the number of flinches) and paw oedema (plethysmometer method) induced by Tsv at doses of 1.0 and 10 mu g/paw for both nociceptive and oedematogenic responses, respectively. Nevertheless, indomethacin (5 mg/kg, i.p.. 30 min prior) was ineffective in altering all of these events. The results of the present study show that Tsv, injected ipl into the rat paw, causes a dose-dependent paw oedema, mechanical hypernociception and flinches (a characteristic biphasic response) in which kinins and NO are substantially involved. Although celecoxib was effective against the oedema and pain caused by Tsv, COX-2 does not seem to be involved in the inflammatory response caused by Tsv. (C) 2008 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 07/04791-1 - Isolamento e caracterização bioquímica da fração responsável pelo efeito pirogênico e nociceptivo presente no veneno do escorpião Tityus serrulatus
• Beneficiário: Glória Emília Petto de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/06373-0 - Isolamento e caracterização bioquímica da fração responsável pelo efeito nociceptivo e pirogênico presente no veneno do escorpião Tityus serrulatus
• Beneficiário: Andréa Carla Pessini
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado