Histological characterization of in vitro adventitious organogenesis in Citrus sinensis

Autor(es)

de Almeida‚ W.A.B. ; de AA Mourao Filho‚ F. ; Mendes‚ B.M.J. ; Rodriguez‚ A.P.M.

• Tipo de documento: Artigo Científico
• Fonte: Biologia Plantarum; v. 50, n. 3, p. 321-325, 2006.

Resumo

The adventitious bud development was induced in epicotyl segments of Valencia sweet orange (Citrus sinensis L. Osbeck). Seeds were cultured in vitro for three weeks in the dark, followed by one week at a 16-h photoperiod. Epicotyl segments were cultured horizontally for the induction of organogenesis in Murashige and Tucker (1969, MT) culture medium supplemented with 1.0 mg dm(-3) benzylaminopurine. Samples were observed by light and scanning electron microscopy from day zero to day 25, when buds were well grown. It was shown that the adventitious buds originated directly from the cambial region on the cut ends of the explants. (AU)

• Processo FAPESP: 99/04073-3 - Optimization of in vitro regeneration systems and establishment of a genetic transformation protocol for Citrus SP
• Beneficiário: Beatriz Madalena Januzzi Mendes
• Modalidade de apoio: Auxílio à Pesquisa - Programa GENOMA