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(Reference retrieved automatically from Google Scholar through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Histological characterization of in vitro adventitious organogenesis in Citrus sinensis

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Author(s):
de Almeida‚ W.A.B. ; de AA Mourao Filho‚ F. ; Mendes‚ B.M.J. ; Rodriguez‚ A.P.M.
Total Authors: 4
Document type: Journal article
Source: Biologia Plantarum; v. 50, n. 3, p. 321-325, 2006.
Abstract

The adventitious bud development was induced in epicotyl segments of Valencia sweet orange (Citrus sinensis L. Osbeck). Seeds were cultured in vitro for three weeks in the dark, followed by one week at a 16-h photoperiod. Epicotyl segments were cultured horizontally for the induction of organogenesis in Murashige and Tucker (1969, MT) culture medium supplemented with 1.0 mg dm(-3) benzylaminopurine. Samples were observed by light and scanning electron microscopy from day zero to day 25, when buds were well grown. It was shown that the adventitious buds originated directly from the cambial region on the cut ends of the explants. (AU)

FAPESP's process: 99/04073-3 - Optimization of in vitro regeneration systems and establishment of a genetic transformation protocol for Citrus sp.
Grantee:Beatriz Madalena Januzzi Mendes
Support Opportunities: Genome Research Grants