Modeling the growth rate and lag time of different strains of Salmonella enterica and Listeria monocytogenes in ready-to-eat lettuce

Autor(es)

Sant'Ana, Anderson S. ^{[1, 2]} ; Franco, Bernadette D. G. M. ^[1] ; Schaffner, Donald W. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, Sao Paulo - Brazil

^[2] Rutgers State Univ, Dept Food Sci, Sch Biol & Environm Sci, New Brunswick, NJ 08903 - USA

• Tipo de documento: Artigo Científico
• Fonte: FOOD MICROBIOLOGY; v. 30, n. 1, p. 267-273, MAY 2012.
• Citações Web of Science: 59

Resumo

The growth parameters (growth rate, mu and lag time, lambda) of three different strains each of Salmonella enterica and Listeria monocytogenes in minimally processed lettuce (MPL) and their changes as a function of temperature were modeled. MPL were packed under modified atmosphere (5% O-2, 15% CO2 and 80% N-2), stored at 7-30 degrees C and samples collected at different time intervals were enumerated for S. enterica and L monocytogenes. Growth curves and equations describing the relationship between mu and lambda as a function of temperature were constructed using the DMFit Excel add-in and through linear regression, respectively. The predicted growth parameters for the pathogens observed in this study were compared to ComBase, Pathogen modeling program (PMP) and data from the literature. High R-2 values (0.97 and 0.93) were observed for average growth curves of different strains of pathogens grown on MPL Secondary models of mu and lambda for both pathogens followed a linear trend with high R2 values (>0.90). Root mean square error (RMSE) showed that the models obtained are accurate and suitable for modeling the growth of S. enterica and L monocytogenes in MP lettuce. The current study provides growth models for these foodborne pathogens that can be used in microbial risk assessment. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 07/54891-2 - Avaliacao quantitativa do risco de salmonella spp e listeria monocytogenes em vegetais minimamente processados.
• Beneficiário: Anderson de Souza Sant'Ana
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/54890-6 - Avaliacao quantitativa do risco de salmonella spp e listeria monocytogenes em vegetais minimamente processados.
• Beneficiário: Bernadette Dora Gombossy de Melo Franco
• Modalidade de apoio: Auxílio à Pesquisa - Regular