Infestin 1R, an intestinal subtilisin inhibitor from Triatoma infestans able to impair mammalian cell invasion by Trypanosoma cruzi

Autor(es)

Lovato, Diogo V. ^[1] ; Amino, Rogerio ^[1] ; Gonzalez, Yamile ^[2] ; Miranda, Antonio ^[3] ; Schenkman, Sergio ^[4] ; Tanaka, Aparecida S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Bioquim, Escola Paulista Med, Sao Paulo - Brazil

^[2] Univ La Habana, Fac Biol, Ctr Estudio Prot, Havana - Cuba

^[3] Univ Fed Sao Paulo, Dept Biofis, Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Experimental Parasitology; v. 129, n. 4, p. 362-367, DEC 2011.
• Citações Web of Science: 4

Resumo

Infestins are Kazal-type serine protease inhibitors described in the midgut of Triatoma infestans, Chagas disease vector. Of all infestins, only infestin 1R (INF1R) does not control host blood coagulation, due to its inhibitory specificity for chymotrypsin-like proteases. We further investigated the effect of INF1R on cell infection by Trypanosoma cruzi. The importance of INF1R reactive site to inhibit T. cruzi cell invasion was confirmed using 1RSFTI, a synthetic cyclic peptide containing the inhibitor reactive site region hybridized to the Sunflower Trypsin Inhibitor-1 (SFTI-1). Our results suggest that INF1R efficiently inhibited parasite cell invasion. For the first time, a serine protease inhibitor, derived from T. infestans, was shown to impair cell invasion by T. cruzi, representing possible new target in parasite cell invasion. (C) 2011 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 09/50434-1 - Estudos moleculares de subtilisinas hipotéticas de Trypanosoma cruzi
• Beneficiário: Diogo Ventura Lovato
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 05/03514-9 - Estudos da função fisiológica e potencial biotecnológico de inibidores de proteases e anti-hemostáticos presentes em artrópodes hematófagos
• Beneficiário: Aparecida Sadae Tanaka
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 02/12593-1 - Caracterização molecular e funcional de inibidores de proteases de estômago de Triatoma infestans
• Beneficiário: Diogo Ventura Lovato
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto