Microcystin-producing genotypes from cyanobacteria in Brazilian reservoirs

Autor(es)

Bittencourt-Oliveira, Maria do Carmo ^{[1, 2]} ; Piccin-Santos, Viviane ^{[1, 2]} ; Gouvea-Barros, Selma ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Sao Paulo - Brazil

^[2] Univ Sao Paulo, ESALQ, Dept Ciencias Biol, BR-13418900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ENVIRONMENTAL TOXICOLOGY; v. 27, n. 8, p. 461-471, AUG 2012.
• Citações Web of Science: 9

Resumo

The aim of this study was to evaluate the use of new oligonucleotide primers (mcyB-F/R, mcyB-F/R-A, and mcyB-F/R-B) designed from Brazilian cyanobacteria for the detection of microcystin-producing genotypes in 27 environmental samples from water reservoirs and 11 strains of Microcystis. Microcystins were found using HPLC in all 11 strains and 19 of the environmental samples. The new oligonucleotide primers amplified fragments of microcystin-producing genes, including the eight environmental samples in which no microcystins were detected by HPLC, but which presented amplified fragments, thereby demonstrating the existence of microcystin-producing genes. The new oligonucleotide primers exhibited better specificity when used with environmental samples and were more reliable in comparison with those described in the literature (mcyB-FAA/RAA and mcyA-Cd/FR), which generate false-negative results. The better performance of these new oligonucleotide primers underline the need for designing molecular markers that are well fitted to the regional biological diversity. As this is a fast predictive technique for determining the presence or absence of microcystins, it could be used either alone or in conjunction with other techniques, such as the screening of samples to be sent for quantitative toxicological analysis using HPLC, thereby reducing monitoring cost and time. (c) 2010 Wiley Periodicals, Inc. Environ Toxicol, 2012. (AU)

• Processo FAPESP: 07/57338-2 - Cianobactérias potencialmente tóxicas de represas de abastecimento público: uma abordagem taxonômica e de saúde pública
• Beneficiário: Viviane Piccin dos Santos
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 06/03878-3 - Monitoramento molecular de cianobactérias hepatotóxicas em sistemas de abastecimento de água na Região Metropolitana de São Paulo
• Beneficiário: Maria Do Carmo Bittencourt de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/52342-6 - Avaliacao de novos marcadores moleculares baseada em genes que codificam para a sintetase de microcistina (mcya e mcyb) e desenvolvimento de testes com pcr para microcystis hepatotoxicas...
• Beneficiário: Selma Gouvêa de Barros
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica