Manganese-enhanced magnetic resonance imaging detects mossy fiber sprouting in the pilocarpine model of epilepsy

Autor(es)

Malheiros, Jackeline M. ^{[1, 2]} ; Polli, Roberson S. ^{[1, 2]} ; Paiva, Fernando F. ^[2] ; Longo, Beatriz M. ^[1] ; Mello, Luiz E. ^[1] ; Silva, Afonso C. ^[3] ; Tannus, Alberto ; Covolan, Luciene ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Physiol, UNIFESP, BR-04023062 Sao Paulo - Brazil

^[2] Univ Sao Paulo, CIERMag Sao Carlos Inst Phys, Sao Carlos, SP - Brazil

^[3] Natl Inst Neurol Disorders & Stroke, Cerebral Microcirculat Unit, Lab Funct & Mol Imaging, NIH, Bethesda, MD - USA

• Tipo de documento: Artigo Científico
• Fonte: Epilepsia; v. 53, n. 7, p. 1225-1232, JUL 2012.
• Citações Web of Science: 18

Resumo

Purpose: Mossy fiber sprouting (MFS) is a frequent finding following status epilepticus (SE). The present study aimed to test the feasibility of using manganese-enhanced magnetic resonance imaging (MEMRI) to detect MFS in the chronic phase of the well-established pilocarpine (Pilo) rat model of temporal lobe epilepsy (TLE). Methods: To modulate MFS, cycloheximide (CHX), a protein synthesis inhibitor, was coadministered with Pilo in a subgroup of animals. In vivo MEMRI was performed 3 months after induction of SE and compared to the neo-Timm histologic labeling of zinc mossy fiber terminals in the dentate gyrus (DG). Key Findings: Chronically epileptic rats displaying MFS as detected by neo-Timm histology had a hyperintense MEMRI signal in the DG, whereas chronically epileptic animals that did not display MFS had minimal MEMRI signal enhancement compared to nonepileptic control animals. A strong correlation (r = 0.81, p < 0.001) was found between MEMRI signal enhancement and MFS. Significance: This study shows that MEMRI is an attractive noninvasive method for detection of mossy fiber sprouting in vivo and can be used as an evaluation tool in testing therapeutic approaches to manage chronic epilepsy. (AU)

• Processo FAPESP: 05/56663-1 - Centro de imagens e espectroscopia in vivo por ressonância magnética para estudo de modelos animais
• Beneficiário: Alberto Tannús
• Modalidade de apoio: Auxílio à Pesquisa - Programa CINAPCE - Temático
• Processo FAPESP: 07/52911-6 - Utilização da técnica de realce por manganês em ressonância magnética no estudo da epilepsia induzida por pilocarpina
• Beneficiário: Jackeline Moraes Malheiros
• Modalidade de apoio: Bolsas no Brasil - Mestrado