| Full text | |
| Author(s): |
Malheiros, Jackeline M.
[1, 2]
;
Polli, Roberson S.
[1, 2]
;
Paiva, Fernando F.
[2]
;
Longo, Beatriz M.
[1]
;
Mello, Luiz E.
[1]
;
Silva, Afonso C.
[3]
;
Tannus, Alberto
;
Covolan, Luciene
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Fed Sao Paulo, Dept Physiol, UNIFESP, BR-04023062 Sao Paulo - Brazil
[2] Univ Sao Paulo, CIERMag Sao Carlos Inst Phys, Sao Carlos, SP - Brazil
[3] Natl Inst Neurol Disorders & Stroke, Cerebral Microcirculat Unit, Lab Funct & Mol Imaging, NIH, Bethesda, MD - USA
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Epilepsia; v. 53, n. 7, p. 1225-1232, JUL 2012. |
| Web of Science Citations: | 18 |
| Abstract | |
Purpose: Mossy fiber sprouting (MFS) is a frequent finding following status epilepticus (SE). The present study aimed to test the feasibility of using manganese-enhanced magnetic resonance imaging (MEMRI) to detect MFS in the chronic phase of the well-established pilocarpine (Pilo) rat model of temporal lobe epilepsy (TLE). Methods: To modulate MFS, cycloheximide (CHX), a protein synthesis inhibitor, was coadministered with Pilo in a subgroup of animals. In vivo MEMRI was performed 3 months after induction of SE and compared to the neo-Timm histologic labeling of zinc mossy fiber terminals in the dentate gyrus (DG). Key Findings: Chronically epileptic rats displaying MFS as detected by neo-Timm histology had a hyperintense MEMRI signal in the DG, whereas chronically epileptic animals that did not display MFS had minimal MEMRI signal enhancement compared to nonepileptic control animals. A strong correlation (r = 0.81, p < 0.001) was found between MEMRI signal enhancement and MFS. Significance: This study shows that MEMRI is an attractive noninvasive method for detection of mossy fiber sprouting in vivo and can be used as an evaluation tool in testing therapeutic approaches to manage chronic epilepsy. (AU) | |
| FAPESP's process: | 05/56663-1 - Magnetic resonance imaging and in vivo spectroscopy center for animal model studies |
| Grantee: | Alberto Tannús |
| Support Opportunities: | Inter-institutional Cooperation in Support of Brain Research (CINAPCE) - Thematic Grants |