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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Evaluation of the schistosomicidal activity of the steroidal alkaloids from Solanum lycocarpum fruits

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Autor(es):
Miranda, Mariza Abreu [1] ; Magalhaes, Lizandra Guidi [2] ; Jorge Tiossi, Renata Fabiane [1] ; Kuehn, Christian Collins [1] ; Rodrigues Oliveira, Luiz Gustavo [1] ; Rodrigues, Vanderlei [3] ; McChesney, James Dewey [4] ; Bastos, Jairo Kenupp [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto FCFRP USP, BR-14040903 Sao Paulo - Brazil
[2] Univ Franca, BR-14404600 Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, BR-14040903 Sao Paulo - Brazil
[4] Arbor Therapeut LLC, Etta, MS 38627 - USA
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Parasitology Research; v. 111, n. 1, p. 257-262, JUL 2012.
Citações Web of Science: 17
Resumo

Solanum lycocarpum (Solanaceae), a Brazilian medicinal plant known as ``wolf fruit,{''} contains about 1.5% of glycoalkaloids in its dried fruits, consisting mainly of solamargine and solasonine. The present work reports the obtainment of the alkaloidic extract of the S. lycocarpum fruit by acid-base extraction and the isolation of the major alkaloid heterosides by chromatographic means, as well as the evaluation of their in vitro schistosomicidal activities. The in vitro schistosomicidal activities of the alkaloidic extract of S. lycocarpum fruits and its isolated steroidal alkaloids were undertaken against adult worms of Schistosoma mansoni. The alkaloidic extract (20, 32, and 50 mu g mL(-1)), solasonine (50 mu M), solamargine (32 and 50 mu M), and equimolar mixture of glycoalkaloids (20, 32, and 50 mu M) lead to the separation of all couple worms and extensive disruption on their teguments, such as sloughing, as well as their deaths within 24 h of incubation. In addition, the alkaloidic extract (10 and 15 mu g mL(-1)), solasonine (50 mu M), solamargine (10, 15, and 20 mu M), and equimolar mixtures of glycoalkaloids (10 and 15 mu M) reduced the development of eggs produced by the adult worms. Solamargine, containing the sugar chain moiety chacotriose, was more active than the solasonine, which contains solatriose sugar chain moiety. A synergistic effect was also observed for a mixture of solamargine and solasonine. Therefore, the alkaloidic extract of S. lycocarpum, and its major components, solamargine and solasonine, showed promising schistosomicidal activity. (AU)

Processo FAPESP: 08/56324-0 - Avaliacao do potencial antiparasitario dos glicoalcaloides dos frutos de solanum lycocarpum.
Beneficiário:Mariza Abreu Miranda
Modalidade de apoio: Bolsas no Brasil - Mestrado