Characterization of two vasoactive peptides isolated from the plasma of the snake Crotalus durissus terrificus

Autor(es)

Barreto, S. A. ^[1] ; Chaguri, L. C. A. G. ^[1] ; Prezoto, B. C. ^[2] ; Lebrun, I. ^[1]

Afiliação do(s) autor(es)

^[1] Butantan Inst, Biochem & Biophys Lab, BR-05503900 Sao Paulo - Brazil

^[2] Butantan Inst, Pharmacol Lab, BR-05503900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOMEDICINE & PHARMACOTHERAPY; v. 66, n. 4, p. 256-265, JUN 2012.
• Citações Web of Science: 4

Resumo

Incubation of plasma from the snake Crotalus durissus terrificus (CDTP) with trypsin generated two hypotensive peptides. The primary structure of the peptides was established for two sequences as: (Ser - Ile - Pro - Gln - Ala - Pro - Thr - Ser - Asn - Leu - Ile - Glu - Ala - Thr - Lys) and (Lys - Pro - Asp - Ala Asn - Gln - Val - Leu - Ile - Gln - Val - Ile - Gly - Val). These peptides display homology with fragments of albumin from Trimeresurus flavoviridis. Bolus intra-arterial injection of the purified or the synthetic peptide produced a strong and sustained vasopressor response in the anaesthetized snake (CDT) and rats (Wistar); this hypotensive effect was also potentiated by captopril-an angiotensin-converting (0.1 mg/kg) enzyme inhibitor. (C) 2012 Elsevier Masson SAS. All rights reserved. (AU)

• Processo FAPESP: 03/09312-3 - Obtenção de peptídeos vasoativos a partir do plasma de serpentes brasileiras (Bothrops jararaca, Crotalus durissus terrificus e Waglerophis merremii)
• Beneficiário: Sandra Alves Barreto Barbosa
• Modalidade de apoio: Bolsas no Brasil - Mestrado