Bupivacaine Injection Leads to Muscle Force Reduction and Histologic Changes in a Murine Model

Autor(es)

McNeill Ingham, Sheila Jean ^{[1, 2]} ; Pochini, Alberto de Castro ^{[1, 3]} ; de Oliveira, Daniel Antonio ^{[1, 3]} ; Garcia Lisboa, Bianca Cristina ^[4] ; Beutel, Abram ^[4] ; Valero-Lapchik, Valderez Bastos ^[4] ; Ferreira, Ana Maria ^[5] ; Abdalla, Rene Jorge ^{[1, 3]} ; Cohen, Moises ^{[1, 3]} ; Han, Sang Won ^{[6, 4]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Orthopaed Surg, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Phys Med & Rehabil, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Ctr Sports Med, Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Ctr Gene Therapy, Sao Paulo - Brazil

^[5] Univ Fed Fluminense, Ctr Med Sci, Sch Vet, Rio De Janeiro - Brazil

^[6] Univ Fed Sao Paulo, Dept Biophys, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PM&R; v. 3, n. 12, p. 1106-1109, DEC 2011.
• Citações Web of Science: 7

Resumo

Objective: To evaluate the effect of bupivacaine on muscle force and histology. We hypothesize that bupivacaine will worsen the muscle's physiological activity. Setting: Controlled laboratory experiment. Methods: Bupivacaine (0.5 mL, 0.5%) was injected into the mid belly and distal portions of the right gastrocnemius in 32 Wistar male rats (the left gastrocnemius was used as a control). After 5, 14, 21, and 28 days, in groups of 4, muscle force was evaluated and the animals were euthanized by an overdose of anesthetic for histologic evaluation. One-way analysis of variance was used to analyze data from force and weight measurements. Only the values of P < .05 were considered to be statistically significant. Results: Bupivacaine causes a process of degeneration-regeneration of the muscle fibers and it also causes a reduction in muscle force, which is significant at 2 and 3 weeks and does not normalize at 4 weeks. The muscle injury is obvious after 5 days, and the degenerative process is predominant at 2 and 3 weeks. We found an increase in muscle mass in the acute phase and a decrease in muscle force. Conclusion: Although our results do not allow a direct clinical application, we believe that caution should be warranted when intramuscular bupivacaine is used. PM R 2011;3:1106-1109 (AU)

• Processo FAPESP: 11/00859-6 - Papel do GM-CSF na reparação da lesão muscular: terapia gênica de lesões musculares
• Beneficiário: Sang Won Han
• Modalidade de apoio: Auxílio à Pesquisa - Regular