Chronic ethanol consumption induces histopathological changes and increases nitric oxide generation in the rat liver

Autor(es)

Tirapelli, Luis F. ^[1] ; Batalhao, Marcelo E. ^[2] ; Jacob-Ferreira, Ana L. ^[1] ; Tirapelli, Daniela P. ^[1] ; Carnio, Evelin C. ^[2] ; Tanus-Santos, Jose E. ^[1] ; Queiroz, Regina H. ^[3] ; Uyemura, Sergio A. ^[3] ; Padovan, Claudia M. ^[4] ; Tirapelli, Carlos R. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, BR-14040902 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Coll Nursing Ribeirao Preto, BR-14040902 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, BR-14040902 Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, BR-14040902 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TISSUE & CELL; v. 43, n. 6, p. 384-391, 2011.
• Citações Web of Science: 9

Resumo

In the present work we evaluated the effect of ethanol consumption in histopathological liver changes and several biochemical biomarkers employed in the detection of hepatic dysfunction. Male Wistar rats were treated with ethanol 20% (vol/vol) for 6 weeks. Histopathological investigation of livers from ethanol-treated animals revealed steatosis. Indices of hepatic function (transaminases) and mitochondrial respiration were not altered in ethanol-treated rats. Chronic ethanol consumption did not alter malondialdehyde (MDA) levels in the liver. Ethanol consumption induced a significant increase on hepatic nitrite and nitrate levels. Treatment with ethanol increased both mRNA expression and immunostaining of iNOS, but not eNOS. Finally, ethanol consumption did not alter hepatic levels of metalloproteinase (MMP)-2 and MMP-9. We conclude that alterations on biochemical biomarkers (nitrite and nitrate levels) and histopathology occurred in ethanol-treated rats, supporting the practice of including both types of evaluation in toxicity studies to detect potential ethanol-related hepatic effects. In our model of ethanol consumption, histopathological liver changes were accompanied by elevation in nitrite and nitrate levels indicating increased nitric oxide (NO) generation. Since iNOS-derived NO contributes to hepatic injury, the increased levels of NO described in our study might contribute to a progressive hepatic damage. Therefore, increases in NO generation may be an early indicator of ethanol-induced liver damage. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 06/60076-7 - Efeito do consumo crônico de etanol sobre o sistema adrenomedulina em aorta e leito mesentérico de ratos e avaliação do papel protetor desse peptídeo nas alterações vasculares induzidas pelo etanol
• Beneficiário: Carlos Renato Tirapelli
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores