Characterization of Primary Isolates of HIV Type 1 CRF28_BF, CRF29_BF, and Unique BF Recombinants Circulating in Sao Paulo, Brazil

Autor(es)

Melo, Fernando Lucas ^[1] ; Jamal, Leda Fatima ^[2] ; de Andrade Zanotto, Paolo Marinho ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Biomed Sci Inst ICB 2, Dept Microbiol, Lab Mol Evolut & Bioinformat, BR-05508000 Sao Paulo - Brazil

^[2] Secretary Hlth Sao Paulo State, Ctr Dis Control, Training & Reference Ctr DST AIDS, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AIDS Research and Human Retroviruses; v. 28, n. 9, p. 882-888, SEP 2012.
• Citações Web of Science: 0

Resumo

We report for the first time the genetic and biological characterization of 10 HIV-1 primary isolates representing CRF28\_BF and CRF29\_BF together with additional unique BF recombinant forms (URFs) obtained by PBMC cocultivation. Recombination is an important factor promoting the increase in the genetic diversity of HIV-1. Notably, more than 20% of HIV-1 sequences worldwide were recombinants. Several recombinant viruses were reported in Brazil, and six circulating recombinant forms (CRFs) have been identified (CRF28\_BF, CRF29\_BF, CRF31\_BC, CRF39\_BF, CRF40\_BF, and CRF46\_BF). CRF28\_BF and CRF29\_BF were found to infect almost 30% of the patients in Sao Paulo State. The near full-length genomes of these 10 primary isolates were amplified by nested PCR in three overlapping segments, purified, and sequenced. Three samples were related to CRF28\_BF, three to CRF29\_BF, and four were unique recombinant forms (URFs), as determined by their breakpoint profile determined with the jpHMM program. Additionally, the coreceptor usage of these isolates was investigated in vitro using GHOST assays, which revealed three dual-tropic (X4/R5) viruses, four lymphotropic (X4) viruses, and three macrophage-tropic (R5) viruses with different V3-loop motifs, which challenges the notion that GWGR-carrying viruses are macrophage-tropic only. In sum, we report a much-anticipated well-characterized panel of viruses representing CRF28\_BF, CRF29\_BF, and URFs from Sao Paulo State, Brazil. (AU)

• Processo FAPESP: 00/04205-6 - Projeto VIRGEN - Viral Genetic Diversity Network (VGDN)
• Beneficiário: Paolo Marinho de Andrade Zanotto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/01554-9 - Caracterização biológica e molecular de recombinantes naturais de HIV-1
• Beneficiário: Fernando Lucas de Melo
• Modalidade de apoio: Bolsas no Brasil - Doutorado