Functional characterization and synergic action of fungal xylanase and arabinofuranosidase for production of xylooligosaccharides

Autor(es):Mostrar menos -

Goncalves, T. A. ^[1] ; Damasio, A. R. L. ^[1] ; Segato, F. ^[1] ; Alvarez, T. M. ^[1] ; Bragatto, J. ^[1] ; Brenelli, L. B. ^[1] ; Citadini, A. P. S. ^[1] ; Murakami, M. T. ^[2] ; Ruller, R. ^[1] ; Paes Leme, A. F. ^[2] ; Prade, R. A. ^[3] ; Squina, F. M. ^[1]

Afiliação do(s) autor(es)

^[1] CNPEM, Lab Nacl Ciencia & Tecnol Bioetanol CTBE, BR-13083970 Campinas, SP - Brazil

^[2] CNPEM, Lab Nacl Biociencias LNBio, BR-13083970 Campinas, SP - Brazil

^[3] Oklahoma State Univ, Dept Microbiol & Mol Genet, Stillwater, OK 74078 - USA

• Tipo de documento: Artigo Científico
• Fonte: Bioresource Technology; v. 119, p. 293-299, SEP 2012.
• Citações Web of Science: 40

Resumo

Plant cell wall degrading enzymes are key technological components in biomass bioconversion platforms for lignocellulosic materials transformation. Cost effective production of enzymes and identification of efficient degradation routes are two economic bottlenecks that currently limit the use of renewable feedstocks through an environmental friendly pathway. The present study describes the hypersecretion of an endo-xylanase (GH11) and an arabinofuranosidase (GH54) by a fungal expression system with potential biotechnological application, along with comprehensive characterization of both enzymes, including spectrometric analysis of thermal denaturation, biochemical characterization and mode of action description. The synergistic effect of these enzymes on natural substrates such as sugarcane bagasse, demonstrated the biotechnological potential of using GH11 and GH54 for production of probiotic xylooligosaccharides from plant biomass. Our findings shed light on enzymatic mechanisms for xylooligosaccharide production, as well as provide basis for further studies for the development of novel enzymatic routes for use in biomass-to-bioethanol applications. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 11/02169-7 - Aspergillus nidulans como modelo para expressão heteróloga de celulases e hemicelulases
• Beneficiário: André Ricardo de Lima Damasio
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/18198-3 - Biologia de sistemas das interações microbianas na decomposição do bagaço de cana
• Beneficiário: Fábio Márcio Squina
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Internacional
• Processo FAPESP: 10/11469-1 - Desenvolvimento de uma biblioteca de enzimas a partir de metagenoma de solo
• Beneficiário: Thabata Maria Alvarez
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto