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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Functional characterization and synergic action of fungal xylanase and arabinofuranosidase for production of xylooligosaccharides

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Author(s):
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Goncalves, T. A. [1] ; Damasio, A. R. L. [1] ; Segato, F. [1] ; Alvarez, T. M. [1] ; Bragatto, J. [1] ; Brenelli, L. B. [1] ; Citadini, A. P. S. [1] ; Murakami, M. T. [2] ; Ruller, R. [1] ; Paes Leme, A. F. [2] ; Prade, R. A. [3] ; Squina, F. M. [1]
Total Authors: 12
Affiliation:
[1] CNPEM, Lab Nacl Ciencia & Tecnol Bioetanol CTBE, BR-13083970 Campinas, SP - Brazil
[2] CNPEM, Lab Nacl Biociencias LNBio, BR-13083970 Campinas, SP - Brazil
[3] Oklahoma State Univ, Dept Microbiol & Mol Genet, Stillwater, OK 74078 - USA
Total Affiliations: 3
Document type: Journal article
Source: Bioresource Technology; v. 119, p. 293-299, SEP 2012.
Web of Science Citations: 40
Abstract

Plant cell wall degrading enzymes are key technological components in biomass bioconversion platforms for lignocellulosic materials transformation. Cost effective production of enzymes and identification of efficient degradation routes are two economic bottlenecks that currently limit the use of renewable feedstocks through an environmental friendly pathway. The present study describes the hypersecretion of an endo-xylanase (GH11) and an arabinofuranosidase (GH54) by a fungal expression system with potential biotechnological application, along with comprehensive characterization of both enzymes, including spectrometric analysis of thermal denaturation, biochemical characterization and mode of action description. The synergistic effect of these enzymes on natural substrates such as sugarcane bagasse, demonstrated the biotechnological potential of using GH11 and GH54 for production of probiotic xylooligosaccharides from plant biomass. Our findings shed light on enzymatic mechanisms for xylooligosaccharide production, as well as provide basis for further studies for the development of novel enzymatic routes for use in biomass-to-bioethanol applications. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 11/02169-7 - Aspergillus nidulans as a model for heterologous expression of cellulases and hemicellulases
Grantee:André Ricardo de Lima Damasio
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 10/11469-1 - Library generation for biomass-conversion enzymes from soil metagenome
Grantee:Thabata Maria Alvarez
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 10/18198-3 - Systems biology of sugarcane bagasse microbial decomposition interactions
Grantee:Fábio Márcio Squina
Support type: Research Grants - Visiting Researcher Grant - International