Functional characterization and synergic action of fungal xylanase and arabinofuranosidase for production of xylooligosaccharides

Author(s):Show less -

Goncalves, T. A. ^[1] ; Damasio, A. R. L. ^[1] ; Segato, F. ^[1] ; Alvarez, T. M. ^[1] ; Bragatto, J. ^[1] ; Brenelli, L. B. ^[1] ; Citadini, A. P. S. ^[1] ; Murakami, M. T. ^[2] ; Ruller, R. ^[1] ; Paes Leme, A. F. ^[2] ; Prade, R. A. ^[3] ; Squina, F. M. ^[1]

Affiliation

^[1] CNPEM, Lab Nacl Ciencia & Tecnol Bioetanol CTBE, BR-13083970 Campinas, SP - Brazil

^[2] CNPEM, Lab Nacl Biociencias LNBio, BR-13083970 Campinas, SP - Brazil

^[3] Oklahoma State Univ, Dept Microbiol & Mol Genet, Stillwater, OK 74078 - USA

• Document type: Journal article
• Source: Bioresource Technology; v. 119, p. 293-299, SEP 2012.
• Web of Science Citations: 40

Abstract

Plant cell wall degrading enzymes are key technological components in biomass bioconversion platforms for lignocellulosic materials transformation. Cost effective production of enzymes and identification of efficient degradation routes are two economic bottlenecks that currently limit the use of renewable feedstocks through an environmental friendly pathway. The present study describes the hypersecretion of an endo-xylanase (GH11) and an arabinofuranosidase (GH54) by a fungal expression system with potential biotechnological application, along with comprehensive characterization of both enzymes, including spectrometric analysis of thermal denaturation, biochemical characterization and mode of action description. The synergistic effect of these enzymes on natural substrates such as sugarcane bagasse, demonstrated the biotechnological potential of using GH11 and GH54 for production of probiotic xylooligosaccharides from plant biomass. Our findings shed light on enzymatic mechanisms for xylooligosaccharide production, as well as provide basis for further studies for the development of novel enzymatic routes for use in biomass-to-bioethanol applications. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 11/02169-7 - Aspergillus nidulans as a model for heterologous expression of cellulases and hemicellulases
• Grantee: André Ricardo de Lima Damasio
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 10/18198-3 - Systems biology of sugarcane bagasse microbial decomposition interactions
• Grantee: Fábio Márcio Squina
• Support Opportunities: Research Grants - Visiting Researcher Grant - International
• FAPESP's process: 10/11469-1 - Library generation for biomass-conversion enzymes from soil metagenome
• Grantee: Thabata Maria Alvarez
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)