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Library generation for biomass-conversion enzymes from soil metagenome

Grant number: 10/11469-1
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): September 01, 2010
Effective date (End): August 31, 2013
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Fábio Márcio Squina
Grantee:Thabata Maria Alvarez
Home Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia, Inovações e Comunicações (Brasil). Campinas , SP, Brazil
Associated research grant:08/58037-9 - Library generation for biomass-conversion enzymes from soil metagenome, AP.BIOEN.JP

Abstract

The gradual shift from petroleum to renewable biomass resources is generally seen as an important contribution to the development of a sustainable industrial society and the effective management of green house emissions. Lignocellulosic materials, such as agricultural and forestry residues, are an abundant and low-cost source of stored energy in the biosphere. Thus, biomass conversion into feedstock sugars has moved towards the forefront of the biofuel industry. However, the saccharification of plant biomass is a complicated and lengthy process, mainly due to the inherent recalcitrance and the complex heterogeneity of the polymers comprising plant cell walls. Lignocellulosic biomass must go through an intensive pretreatment step, after which enzymes are used to break down the polysaccharides biomass into simple sugar suitable for fermentation and ethanol production. Likewise, enzymatic conversion of cellulose and hemicellulose into simple sugar is also a demanding task, where a consortium of enzymes is needed for complete saccharification of these polysaccharides. Aiming at the entire exploitation of the plant cell wall polysaccharides, as an environmentally renewable energy source, an extensive repertoire of hydrolytic enzymes would play a major role for biofuel production. The objective of this proposal is the generation of a toolkit of lignocellulolytic enzymes with a wide range of biotechnological applications, including their use as players for the development of strategies for second generation ethanol production. The prospection of these enzymes will be done from soil metagenome, which contemplates a pioneering strategy towards the prospection of biomass conversion enzymes from microorganisms not conventionally cultivable. Additionally, this study may intensively contribute to the development of the field of bioenergy by improving techniques in rapid detection of hydrolysis using capillary electrophoresis and implementing heterologous gene expression in filamentous fungi. (AU)

Scientific publications (9)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PIMENTEL, AGNES C.; EMATSU, GABRIELA C. G.; LIBERATO, MARCELO V.; PAIXAO, DOUGLAS A. A.; FRANCO CAIRO, JOAO PAULO L.; MANDELLI, FERNANDA; TRAMONTINA, ROBSON; GANDIN, CESAR A.; DE OLIVEIRA NETO, MARIO; SQUINA, FABIO M.; ALVAREZ, THABATA M. Biochemical and biophysical properties of a metagenome-derived GH5 endoglucanase displaying an unconventional domain architecture. International Journal of Biological Macromolecules, v. 99, p. 384-393, JUN 2017. Web of Science Citations: 3.
CAMPOS, BRUNA MEDEIA; LIBERATO, MARCELO VIZONA; ALVAREZ, THABATA MARIA; ZANPHORLIN, LETICIA MARIA; EMATSU, GABRIELA CRISTINA; BARUD, HERNANE; POLIKARPOV, IGOR; RULLER, ROBERTO; GILBERT, HARRY J.; DE MATTOS ZERI, ANA CAROLINA; SQUINA, FABIO MARCIO. A Novel Carbohydrate-binding Module from Sugar Cane Soil Metagenome Featuring Unique Structural and Carbohydrate Affinity Properties. Journal of Biological Chemistry, v. 291, n. 45, p. 23734-23743, NOV 4 2016. Web of Science Citations: 5.
FRANCO CAIRO, JOAO P. L.; CARAZZOLLE, MARCELO F.; LEONARDO, FLAVIA C.; MOFATTO, LUCIANA S.; BRENELLI, LIVIA B.; GONCALVES, THIAGO A.; UCHIMA, CRISTIANE A.; DOMINGUES, ROMANIA R.; ALVAREZ, THABATA M.; TRAMONTINA, ROBSON; VIDAL, RAMON O.; COSTA, FERNANDO F.; COSTA-LEONARDO, ANA M.; PAES LEME, ADRIANA F.; PEREIRA, GONCALO A. G.; SQUINA, FABIO M. Expanding the Knowledge on Lignocellulolytic and Redox Enzymes of Worker and Soldier Castes from the Lower Termite Coptotermes gestroi. FRONTIERS IN MICROBIOLOGY, v. 7, OCT 13 2016. Web of Science Citations: 7.
ALVAREZ, THABATA MARIA; LIBERATO, MARCELO VIZONA; FRANCO CAIRO, JOO PAULO L.; PAIXO, DOUGLAS A. A.; CAMPOS, BRUNA M.; FERREIRA, MARCEL R.; ALMEIDA, RODRIGO F.; PEREIRA, ISABELA O.; BERNARDES, AMANDA; EMATSU, GABRIELA C. G.; CHINAGLIA, MARIANA; POLIKARPOV, IGOR; NETO, MARIO DE OLIVEIRA; SQUINA, FABIO MARCIO. A Novel Member of GH16 Family Derived from Sugarcane Soil Metagenome. Applied Biochemistry and Biotechnology, v. 177, n. 2, p. 304-317, SEP 2015. Web of Science Citations: 2.
ALVAREZ, THABATA M.; PAIVA, JOICE H.; RUIZ, DIEGO M.; CAIRO, JOAO PAULO L. F.; PEREIRA, ISABELA O.; PAIXAO, DOUGLAS A. A.; DE ALMEIDA, RODRIGO F.; TONOLI, CELISA C. C.; RULLER, ROBERTO; SANTOS, CAMILA R.; SQUINA, FABIO M.; MURAKAMI, MARIO T. Structure and Function of a Novel Cellulase 5 from Sugarcane Soil Metagenome. PLoS One, v. 8, n. 12 DEC 17 2013. Web of Science Citations: 19.
FRANCO CAIRO, JOAO PAULO L.; OLIVEIRA, LEANDRO C.; UCHIMA, CRISTIANE A.; ALVAREZ, THABATA M.; CITADINI, ANA PAULA DA S.; COTA, JUNIO; LEONARDO, FLAVIA COSTA; COSTA-LEONARDO, ANA M.; CARAZZOLLE, MARCELO F.; COSTA, FERNANDO F.; PEREIRA, GONCALO A. G.; SQUINA, FABIO M. Deciphering the synergism of endogenous glycoside hydrolase families 1 and 9 from Coptotermes gestroi. Insect Biochemistry and Molecular Biology, v. 43, n. 10, p. 970-981, OCT 2013. Web of Science Citations: 15.
ALVAREZ, THABATA M.; GOLDBECK, ROSANA; DOS SANTOS, CAMILA RAMOS; PAIXAO, DOUGLAS A. A.; GONCALVES, THIAGO A.; FRANCO CAIRO, JOAO PAULO L.; ALMEIDA, RODRIGO FERREIRA; PEREIRA, ISABELA DE OLIVEIRA; JACKSON, GEORGE; COTA, JUNIO; BUECHLI, FERNANDA; CITADINI, ANA PAULA; RULLER, ROBERTO; POLO, CARLA CRISTINA; NETO, MARIO DE OLIVEIRA; MURAKAMI, MARIO T.; SQUINA, FABIO M. Development and Biotechnological Application of a Novel Endoxylanase Family GH10 Identified from Sugarcane Soil Metagenome. PLoS One, v. 8, n. 7 JUL 29 2013. Web of Science Citations: 12.
GONCALVES, T. A.; DAMASIO, A. R. L.; SEGATO, F.; ALVAREZ, T. M.; BRAGATTO, J.; BRENELLI, L. B.; CITADINI, A. P. S.; MURAKAMI, M. T.; RULLER, R.; PAES LEME, A. F.; PRADE, R. A.; SQUINA, F. M. Functional characterization and synergic action of fungal xylanase and arabinofuranosidase for production of xylooligosaccharides. Bioresource Technology, v. 119, p. 293-299, SEP 2012. Web of Science Citations: 39.
FRANCO CAIRO, JOAO PAULO L.; LEONARDO, FLAVIA C.; ALVAREZ, THABATA M.; RIBEIRO, DANIELA A.; BUECHLI, FERNANDA; COSTA-LEONARDO, ANA M.; CARAZZOLLE, MARCELO F.; COSTA, FERNANDO F.; PAES LEME, ADRIANA F.; PEREIRA, GONCALO A. G.; SQUINA, FABIO M. Functional characterization and target discovery of glycoside hydrolases from the digestome of the lower termite Coptotermes gestroi. BIOTECHNOLOGY FOR BIOFUELS, v. 4, NOV 14 2011. Web of Science Citations: 22.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
THABATA MARIA ALVAREZ. Library generation for biomass conversion enzymes from soil metagenome. 2013. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.