Functional and structural characterization of lipolytic enzymes identified in a metagenomic library of consortium specialized on diesel oil degradation

• Grant number: 13/03568-8
• Support Opportunities: Regular Research Grants
• Duration: August 01, 2014 - July 31, 2016
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Eliana Gertrudes de Macedo Lemos
• Grantee: Eliana Gertrudes de Macedo Lemos
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Microorganisms and microbial enzymes have long been used for processing natural materials. Currently, in the manufacture, in the food industry and in the detergents production, enzymes serve as reactions catalysts. Lipolytic enzymes have been attracting global market attention because of their enormous biotechnological potential, as for example: in the enzymatic mixtures for detergents production; in the processing of leather for the removal of hair and subcutaneous fat; in the cosmetic, perfumes and other pharmaceuticals production, and biodiesel. Most lipolytic enzymes are derived from microbes, present low toxicity, are easily biodegraded and are notably selective of chemicals, a very positive trait which permits pharmaceutical products made with these enzymes to have few collateral effects. Researches on microbe diversity affirmed that the majority of the microorganisms that composes the biosphere was not studied yet, thus, in order to access the genetic material of microbe species, the metagenomic became a boarding that offers almost unlimited combination to find new genes coders of excellent products as lipases and esterases. Inside this context, it was constructed a metagenomic library of DNA consortia specialized in diesel oil degradation in the Laboratory of Biochemistry of Microorganisms and Plants (LBMP), UNESP, Jaboticabal Campus. In this library, genes of biotechnological interest have been selected, among which, those that codify lipases and esterases. Thus, this work aims to produce on a large scale, to determine the enzymatic activity and to crystallize enzymes identified from the analysis of the open reading frames, because of the high biotechnological potential that this preliminary characterization disclosed. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MAESTER, THAIS CARVALHO; PEREIRA, MARIANA RANGEL; MACHADO SIERRA, E. G.; BALAN, ANDREA; DE MACEDO LEMOS, ELIANA GERTRUDES. Characterization of EST3: a metagenome-derived esterase with suitable properties for biotechnological applications. Applied Microbiology and Biotechnology, v. 100, n. 13, p. 5815-5827, JUL 2016. (11/09064-6, 13/03568-8)

MAESTER, THAIS CARVALHO; PEREIRA, MARIANA RANGEL; MALAMAN, ALIANDRA M. GIBERTONI; BORGES, JANAINA PIRES; PEREIRA, PAMELA APARECIDA MALDANER; LEMOS, ELIANA G. M.. Exploring Metagenomic Enzymes: A Novel Esterase Useful for Short-Chain Ester Synthesis. CATALYSTS, v. 10, n. 10, OCT 2020. (11/09064-6, 13/03568-8)