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"Prospecting, functional and structural characterization of lipolytic enzymes of marine metagenomes"

Abstract

Lipolitic enzymes are interesting biocatalysts due to the different and important uses that they can perform in a several industry areas such as: production of detergents and pharmaceutical processing, synthesis of esters and peptides, transesterification, biosurfactants production and the resolution of racemic mixtures to generate optically active compounds. Microbes are of great interest to the biotechnology and industrial sector, due to the possibility of having their biological molecules with biotechnological appeal being produced in large scale and low cost, exhibiting stability and improved catalytic properties, mainly those originated from adverse environment. The marine environment with it's typical and differentiated characteristics related to salinity and temperature allow a wider possibility to the development of new and more efficient production processes. Thus, this project aims to find other lipases from the marine environment based on evaluation of this particular ecosystem and at the same time, to use biological material already present at the LBMP. We have recently described in the literature a particular lipase included in the genome of a marine bacteria (Marinobacter adhaerens) obtained through the reconstruction of this particular gene based on the genome found in the Web deposited by researchers of Tara Ocean Expedition (2003 - 2010). We intend to clone and express this gene and further to characterize biochemically and functionally this enzyme. Besides this particular situation, some 200 metagenomics' samples were already collected from marine areas from different regions of the world, which should allow us to find other equally important enzymes. (AU)

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