Effect of annexin-A1 peptide treatment during lung inflammation induced by lipopolysaccharide

Autor(es)

da Cunha, Esther Emanuella ^[1] ; Oliani, Sonia Maria ^[1] ; Damazo, Amilcar Sabino ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Fed Univ Mato Grosso UFMT, Fac Med, Dept Basic Sci Hlth, BR-78060900 Mato Grosso, MT - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Pulmonary Pharmacology & Therapeutics; v. 25, n. 4, p. 303-311, AUG 2012.
• Citações Web of Science: 8

Resumo

Lung endotoxemia is characterized by neutrophil accumulation, increased vascular permeability and parenchymal injury. This can also affect the endogenous pathways that operate in the host to keep inflammation under control. Here, we demonstrate differential expression of annexin-A1 (AnxA1) protein in mice after the local or intraperitoneal administration of lipopolysaccharide (LPS; 1 mg/kg) in mice and the regulation of the endotoxemic inflammation after the pre-treatment with the AnxA1 peptidomimetic Ac2-26. The intranasal administration of LPS induced the leukocyte migration and cytokine release to the alveolar space, whereas the peritoneal administration of LPS generated a deregulated cellular and cytokine response, with a marked degree of leukocyte adhesion in the microcirculation. The peptide Ac2-26 pre-treatment inhibited the leukocyte migration and the pro-inflammatory cytokine release. Also, it induced the expression of endogenous AnxA1 and the antiinflammatory cytokine IL-10. In conclusion, our data obtained from endotoxemia induced by local or intraperitoneal LPS administration suggested that the molecular mechanisms induced by AnxAl peptidomimetic Ac2-26 lead to the regulation of leukocyte activation/migration and cytokine production induced by LPS. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 07/00577-5 - Efeito protetor da proteina anexina-a1 durante a endotoxemia experimental induzida por lipopolissacarideos em camundongos.
• Beneficiário: Esther Emanuella da Cunha
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 05/56855-8 - Efeito da proteína antiinflamatória anexina 1 na fibrose pulmonar induzida pela bleomicina
• Beneficiário: Amílcar Sabino Damazo
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores