High frequency of immature dendritic cells and altered in situ production of interleukin-4 and tumor necrosis factor-ALPHA in lung cancer

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Baleeiro, R. B. ; Anselmo, L. B. ; Soares, F. A. ; Pinto, C. A. L. ; Ramos, O. ; Gross, J. L. ; Haddad, F. ; Younes, R. N. ; Tomiyoshi, M. Y. ; Bergami-Santos, P. C. ; Barbuto, J. A. M. ^[11]

• Tipo de documento: Artigo Científico
• Fonte: CANCER IMMUNOLOGY IMMUNOTHERAPY; v. 57, n. 9, p. 1335-1345, Sept. 2008.
• Área do conhecimento: Ciências Biológicas - Imunologia
• Assunto(s): Neoplasias pulmonares Fatores de necrose tumoral Células dendríticas Interleucina-4 Imuno-histoquímica

Resumo

Antigen-presenting cells, like dendritic cells (DCs) and macrophages, play a significant role in the induction of an immune response and an imbalance in the proportion of macrophages, immature and mature DCs within the tumor could affect significantly the immune response to cancer. DCs and macrophages can differentiate from monocytes, depending on the milieu, where cytokines, like interleukin (IL)-4 and granulocyte-macrophage colony-stimulating factor (GM-CSF) induce DC differentiation and tumor necrosis factor (TNF)-ALPHA induce DC maturation. Thus, the aim of this work was to analyze by immunohistochemistry the presence of DCs (S100+ or CD1a+), macrophages (CD68+), IL-4 and TNF-ALPHA within the microenvironment of primary lung carcinomas. Higher frequencies of both immature DCs and macrophages were detected in the tumor-affected lung, when compared to the non-affected lung. Also, TNF-ALPHA-positive cells were more frequent, while IL-4-positive cells were less frequent in neoplastic tissues. This decreased frequency of mature DCs within the tumor was further confirmed by the lower frequency of CD14-CD80+ cells in cell suspensions obtained from the same lung tissues analyzed by flow cytometry. These data are discussed and interpreted as the result of an environment that does not oppose monocyte differentiation into DCs, but that could impair DC maturation, thus affecting the induction of effective immune responses against the tumor. (AU)

• Processo FAPESP: 05/50422-2 - Estudo da expressao de citocinas no microambiente tumoral em pacientes portadores de neoplasias pulmonares e sua co-relacao com a presenca de macrofagos e celulas dendriticas.
• Beneficiário: Renato Brito Baleeiro
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 04/09956-0 - Modulação funcional de células dentríticas em diferentes situações fisiopatológicas
• Beneficiário: Jose Alexandre Marzagão Barbuto
• Modalidade de apoio: Auxílio à Pesquisa - Temático