Combined fish oil and astaxanthin supplementation modulates rat lymphocyte function

Autor(es)

Otton, Rosemari ^[1] ; Marin, Douglas Popp ^[1] ; Bolin, Anaysa Paola ^[1] ; Santos Macedo, Rita de Cassia ^[1] ; Campoio, Thais Regina ^[1] ; Fineto, Jr., Claudio ^[1] ; Guerra, Beatriz Alves ^[1] ; Leite, Jose Roberto ^[2] ; Barros, Marcelo Paes ^{[1, 3]} ; Mattei, Rita ^[2]

Afiliação do(s) autor(es)

^[1] Univ Cruzeiro Sul, CBS, Postgrad Program, BR-03342000 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo UNIFESP, Dept Psicobiol, BR-04023062 Sao Paulo - Brazil

^[3] Univ Cruzeiro Sul, Human Movement Sci Inst Phys Act & Sport Sci, Postgrad Program, BR-01506000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: EUROPEAN JOURNAL OF NUTRITION; v. 51, n. 6, p. 707-718, SEP 2012.
• Citações Web of Science: 14

Resumo

Higher intakes of n-3 polyunsaturated fatty acids that are abundant in marine fishes have been long described as a ``good nutritional intervention{''} with increasing clinical benefits to cardiovascular health, inflammation, mental, and neurodegenerative diseases. The present study was designed to investigate the effect of daily fish oil (FO-10 mg EPA/kg body weight (BW) and 7 mg DHA/kg BW) intake by oral gavage associated with the antioxidant astaxanthin (ASTA-1 mg/kg BW) on the redox metabolism and the functional properties of lymphocytes from rat lymph nodes. This study was conducted by measurements of lymphocyte proliferation capacity, ROS production {[}superoxide (O (2) (aEuro cent a') ) and hydrogen peroxide (H2O2)], nitric oxide (NOaEuro cent) generation, intracellular calcium release, oxidative damage to lipids and proteins, activities of major antioxidant enzymes, GSH/GSSG content, and cytokines release. After 45 days of FO + ASTA supplementation, the proliferation capacity of activated T- and B-lymphocytes was significantly diminished followed by lower levels of O (2) (aEuro cent a') , H2O2 and NOaEuro cent production, and increased activities of total/SOD, GR and GPx, and calcium release in cytosol. ASTA was able to prevent oxidative modification in cell structures through the suppression of the oxidative stress condition imposed by FO. l-selectin was increased by FO, and IL-1 beta was decreased only by ASTA supplementation. We can propose that association of ASTA with FO could be a good strategy to prevent oxidative stress induced by polyunsaturated fatty acids and also to potentiate immuno-modulatory effects of FO. (AU)

• Processo FAPESP: 09/12342-8 - Ação do carotenóide astaxantina na regulação do equilíbrio redox no músculo esquelético de ratos sob atividade física exaustiva (natação): projeto AstaSwim
• Beneficiário: Andréa Ramalho Mihaliuc
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 02/09405-9 - Estudo da atividade antioxidante da astaxantina em lipossomos unilamelares: bloqueando radicais livres e afetando a permeabilidade da membrana?
• Beneficiário: Marcelo Paes de Barros
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 07/03334-6 - Avaliação dos efeitos anti-oxidantes da astaxantina e seu papel regulador na funcionalidade de neutrófilos e linfócitos de diabéticos
• Beneficiário: Rosemari Otton
• Modalidade de apoio: Auxílio à Pesquisa - Regular