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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Combined fish oil and astaxanthin supplementation modulates rat lymphocyte function

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Author(s):
Otton, Rosemari [1] ; Marin, Douglas Popp [1] ; Bolin, Anaysa Paola [1] ; Santos Macedo, Rita de Cassia [1] ; Campoio, Thais Regina [1] ; Fineto, Jr., Claudio [1] ; Guerra, Beatriz Alves [1] ; Leite, Jose Roberto [2] ; Barros, Marcelo Paes [1, 3] ; Mattei, Rita [2]
Total Authors: 10
Affiliation:
[1] Univ Cruzeiro Sul, CBS, Postgrad Program, BR-03342000 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo UNIFESP, Dept Psicobiol, BR-04023062 Sao Paulo - Brazil
[3] Univ Cruzeiro Sul, Human Movement Sci Inst Phys Act & Sport Sci, Postgrad Program, BR-01506000 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: EUROPEAN JOURNAL OF NUTRITION; v. 51, n. 6, p. 707-718, SEP 2012.
Web of Science Citations: 14
Abstract

Higher intakes of n-3 polyunsaturated fatty acids that are abundant in marine fishes have been long described as a ``good nutritional intervention{''} with increasing clinical benefits to cardiovascular health, inflammation, mental, and neurodegenerative diseases. The present study was designed to investigate the effect of daily fish oil (FO-10 mg EPA/kg body weight (BW) and 7 mg DHA/kg BW) intake by oral gavage associated with the antioxidant astaxanthin (ASTA-1 mg/kg BW) on the redox metabolism and the functional properties of lymphocytes from rat lymph nodes. This study was conducted by measurements of lymphocyte proliferation capacity, ROS production {[}superoxide (O (2) (aEuro cent a') ) and hydrogen peroxide (H2O2)], nitric oxide (NOaEuro cent) generation, intracellular calcium release, oxidative damage to lipids and proteins, activities of major antioxidant enzymes, GSH/GSSG content, and cytokines release. After 45 days of FO + ASTA supplementation, the proliferation capacity of activated T- and B-lymphocytes was significantly diminished followed by lower levels of O (2) (aEuro cent a') , H2O2 and NOaEuro cent production, and increased activities of total/SOD, GR and GPx, and calcium release in cytosol. ASTA was able to prevent oxidative modification in cell structures through the suppression of the oxidative stress condition imposed by FO. l-selectin was increased by FO, and IL-1 beta was decreased only by ASTA supplementation. We can propose that association of ASTA with FO could be a good strategy to prevent oxidative stress induced by polyunsaturated fatty acids and also to potentiate immuno-modulatory effects of FO. (AU)

FAPESP's process: 09/12342-8 - The carotenoid astaxanthin as a redox switch in muscle tissues of rats submitted to exhaustive swimming exercise: the AstaSwim project
Grantee:Andréa Ramalho Mihaliuc
Support Opportunities: Scholarships in Brazil - Master